Tanshinone iia

Manufactured by Selleck Chemicals

Sourced in United States

Tanshinone IIA is a naturally occurring compound isolated from the root of the Salvia miltiorrhiza plant. It is a common laboratory reagent used in biochemical and pharmacological research. Tanshinone IIA is known for its various biological activities, including anti-inflammatory, antioxidant, and cardioprotective properties. This compound is frequently utilized in experiments investigating its potential therapeutic applications.

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4 protocols using tanshinone iia

Antibody Profiling for Metabolic Regulation

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Polyclonal C/EBP-α antibody, monoclonal PPAR-γ antibody, monoclonal STAT-3 antibody, monoclonal phospho-STAT-3 (p-STAT-3) antibody, polyclonal STAT-5 antibody, and polyclonal phospho-STAT-5 (p-STAT-5) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal p-HSL (S563), polyclonal p-HSL (S565) and polyclonal p-HSL (S660) were purchased from Cell Signaling Technology (Danvers, MA, USA). Polyclonal HSL antibody was purchased from Cayman chemical. (Ann Arbor, MI, USA). Monoclonal FAS antibody was purchased from BD Bioscience (San Jose, CA, USA). The active compound Tanshinone IIA was purchased from Selleckchem (Houston, TX, USA). Monoclonal β-actin antibody was purchased from Sigma (St. Louis, MO, USA). Polyclonal perilipin A antibody was purchased from Bio Vision Inc. (Milpitas, CA, USA).

Park Y.K., Obiang-Obounou B.W., Lee J., Lee T.Y., Bae M.A., Hwang K.S., Lee K.B., Choi J.S, & Jang B.C. (2017). Anti-Adipogenic Effects on 3T3-L1 Cells and Zebrafish by Tanshinone IIA. International Journal of Molecular Sciences, 18(10), 2065.

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Investigating NSCLC Cell Signaling Pathways

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Human NSCLC cells, including HCC827, H1975, and A549, and the immortalized lung epithelial cells HBE and NL20, immortalized lung fibroblast cell MRC5, were obtained from American Type Culture Collection (ATCC, Manassas, VA). All cells were maintained at the incubator according to the standard protocols and subjected to routinely checking for mycoplasma contamination. Antibodies against p-EGFR (#3777), p-Akt (#4060), p-ERK1/2 (#4370), VDAC1 (#4866), cleaved-PARP (#5625), cleaved-caspase 3 (#9664), Mcl-1 (#94296), Bcl-xL (#2764), Bcl-2 (#4223), VDAC1 (#4661), Bax (#14796), Cytochrome c (#4280), β-actin (#3700), Akt (#2920), ubiquitin (#3936), and α-Tubulin (#2144) were purchased from Cell Signaling Technology, Inc. (Beverly, MA). The natural product Tanshinone IIA (>99%), PD98059, and LY294002 were purchased from Selleck Chemicals (Houston, TX). Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, MA) was used for transient transfection following the manufacturer’s instructions.

Gao F., Li M., Liu W, & Li W. (2020). Inhibition of EGFR Signaling and Activation of Mitochondrial Apoptosis Contribute to Tanshinone IIA-Mediated Tumor Suppression in Non-Small Cell Lung Cancer Cells. OncoTargets and therapy, 13, 2757-2769.

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Tanshinone IIA Effects on HK1 Cell Line

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Human NPC cell line HK1 was obtained from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. HK1 cells were seeded in DMEM (cat. no. C11995500BT; Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (cat. no. P30-3301; PAN-Biotech) and 1% penicillin-streptomycin (cat. no. 15140-122; Gibco; Thermo Fisher Scientific, Inc.) in a water-saturated atmosphere at 37°C. Tanshinone IIA (Fig. 1A) was procured from Selleck Chemicals (cat. no. S2365). Dimethyl sulfoxide (cat. no. 276855; Sigma-Aldrich; Merck KGaA) was used as a negative control.

Wang Y., Jin W, & Wang J. (2021). Tanshinone IIA regulates microRNA-125b/foxp3/caspase-1 signaling and inhibits cell viability of nasopharyngeal carcinoma. Molecular Medicine Reports, 23(5), 371.

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Colorectal Cancer Cell Line Characterization

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Human colorectal cancer cell line HCT-116 (ATCC, USA) was cultured in 1640 medium and LoVo (ATCC, USA) was cultured in F-12K medium, at 37°C in a 5% CO 2 incubator, containing 10% fetal bovine serum (FBS), 100U/mL penicillin, 100mg/mL streptomycin. Rabbit monoclonal antibodies against human Ecadherin, N-cadherin, Vimentin, Snail, β-arrestin1, GSK-3β, β-catenin, c-Myc and CyclinD1 were purchased from Cell Signaling Technology (USA). Rabbit monoclonal antibodies against human MMP2 and MMP9 were purchased from Santa Cruz Biotechnology (USA). Mouse monoclonal antibodies against human GAPDH were purchased from Proteintech (China). Tanshinone IIA was purchased from Selleck (Houston, TX, USA).

Song Q., Yang L., Han Z., Wu X., Li R., Zhou L., Liu N., Sui H., Cai J., Wang Y., Ji Q., & Li Q. (2020). Tanshinone IIA Inhibits Epithelial-to-mesenchymal Transition Through Regulating β-arrestin1 Mediated β-catenin Signaling Pathway in Colorectal Cancer.

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