Ha tag 6e2 mouse antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The HA-Tag (6E2) mouse antibody is a monoclonal antibody that recognizes the hemagglutinin (HA) tag, a commonly used epitope tag. The antibody can be used to detect and immunoprecipitate HA-tagged proteins.

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Lab products found in correlation

Lipofectamine reagent, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

HA-Tag (6E2) mouse monoclonal antibody Anti-HA-tag (6E2) mouse monoclonal antibody HA-Tag (6E2; HA-tag antibody HA-tag

3 protocols using ha tag 6e2 mouse antibody

HA-LMO4 Plasmid Transfection Protocol

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HA-LMO4 plasmids were generated/isolated using the mammalian expression vector pRK5 (cat. no. 22964, Addgene, Cambridge, MA, USA). UBOC1 cells at 50–60% confluence were transfected with HA-tagged LMO4 using Lipofectamine reagent (Invitrogen, Carlsbad, CA, USA). Transfection of the plasmid DNA was verified by immunoblotting with HA-Tag (6E2) mouse antibody (Cell Signaling, Danvers, MA, USA). The transfected cells were cultured for 48 h and then used for the experiments.

Rathinam R., Ghosh S., Neumann W, & Jamesdaniel S. (2015). Cisplatin-induced apoptosis in auditory, renal, and neuronal cells is associated with nitration and downregulation of LMO4. Cell Death Discovery, 1, 15052-.

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Overexpression of HA-tagged LMO4

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HA-LMO4 plasmids were generated/isolated using the mammalian expression vector pRK5 (Cat. No. 22964, Addgene, Cambridge, MA). UBOC1 cells at 50–60% confluence were transfected with HA-tagged LMO4 using lipofectamine reagent (Invitrogen, Carlsbad, CA). Transfection of the plasmid DNA was verified by immunoblotting with HA-Tag (6E2) mouse antibody (Cell Signaling, Danvers, MA). The transfected cells were cultured for 48 h and then used for the experiments.

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Overexpression of LMO4 in UBOC1 Cells

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Mammalian expression vector pRK5 (catalog no. 22964, Addgene, Cambridge, MA) was used for the overexpression of LMO4, following the manufacturer's protocol. UBOC1 cells were transfected with HA-tagged LMO4 using lipofectamine reagent (Invitrogen, Carlsbad, CA) at 50–60% confluence and cultured for 48 h. Transfection of the plasmid DNA was verified by immunoblotting with HA-Tag (6E2) mouse antibody (Cell Signaling, Danvers, MA) and overexpression of LMO4 was verified by immunoblotting with anti-LMO4 [35] .

Jamesdaniel S., Rathinam R, & Neumann W.L. (2016). Targeting nitrative stress for attenuating cisplatin-induced downregulation of cochlear LIM domain only 4 and ototoxicity. Redox Biology, 10, 257-265.

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