Mice were sacrificed immediately after the end of a 6-min FST to minimize any effects due to the minimal stress manipulation (Fig.1a ). Fresh brains were extracted and processed for morphological analysis following the FD Rapid GolgiStain protocol (PK401, FD Neurotechnologies, Inc, Columbia, US) with few changes. When extracted, brains were coded for quantitative analysis. Both hemispheres were used to obtain 100μm sections using a vibratome. Sections were collected serially, dehydrated, cleared in xylene, and then cover-slipped.
Fd rapid golgistain protocol
Manufactured by FD NeuroTechnologies
Sourced in United States
The FD Rapid GolgiStain protocol is a laboratory technique used for the rapid and efficient staining of neuronal structures within tissue samples. This protocol allows for the visualization of the detailed morphology of individual neurons, including their dendritic and axonal processes. The FD Rapid GolgiStain provides a comprehensive and high-quality staining method for a wide range of neurobiological research applications.
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3 protocols using fd rapid golgistain protocol
1
Rapid Golgi Staining of Mouse Brains
2
Golgi Staining for Dendritic Spine Analysis
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Golgi stain was also used to analyze dendrite spines. The procedure followed FD Rapid GolgiStain protocol (FD NeuroTechnologies Inc., Columbia, MD, USA). Brains were sectioned on a cryostat with 100-µm thickness and mounted on gelatin-coated microscope slides. The slides were cleared in xylene and cover-slipped with Permount mounting media (Thermo Fisher Scientific, Waltham, MA, USA). To measure spine density, periinfarct cortex and hippocampus from each animal were selected. Images were observed with a 100×oil immersion objective lens by Image-Pro Plus Image analyzer. The spine number was expressed as the number of spines/10 µm.
3
Rapid Golgi Staining of Mouse Brains
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