Fluorescently conjugated protein or antibodies used for cell-surface staining and intracellular staining for cytokines and transcription factors are listed in Supplementary Table 2 . α-GalCer (KRN7000) was purchased from Enzo. CD1d-PBS57 (conjugated with either PE or allophycocyanin) was obtained from the tetramer facility of the US National Institutes of Health. Collagenase IV used for tissue digestion was purchased from Sigma. Taq Master Mix was purchased from Vazyme Biotech. A mouse IFN-γ ELISA kit and a mouse IL-4 ELISA kit were obtained from BioLegend. PMA and ionomycin were obtained from Merck. Stem cell factor, murine IL-3, and murine IL-6 were obtained from PeproTech. Met-RANTES was purchased from R&D Systems. DAPI was obtained from Cell Signaling Technology.
Mouse il 4 elisa kit
Manufactured by BioLegend
The Mouse IL-4 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of mouse interleukin-4 (IL-4) in cell culture supernatants, serum, and plasma.
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Lab products found in correlation
α galcer, by Enzo Life Sciences (1 mentions)
Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Cell Signaling Technology (1 mentions)
Mouse ifn γ elisa kit, by BioLegend (1 mentions)
Murine il 3, by Thermo Fisher Scientific (1 mentions)
Murine il 6, by Thermo Fisher Scientific (1 mentions)
Met rantes, by R&D Systems (1 mentions)
Collagenase 4, by Merck Group (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Taq master mix, by Vazyme (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Ffa quantification colorimetric fluorometric kit, by Abcam (1 mentions)
2 protocols using mouse il 4 elisa kit
1
Multiparameter Flow Cytometry Analysis
2
Adipose Tissue Biomarker Quantification
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The subcutaneous WAT (scWAT) was homogenized in tissue protein extraction reagent. Total protein was extracted and the concentration was measured using BCA protein quantification kit. The content of cytokines and norepinephrine in local scWAT were determined by ELISA kits. IL-4 was measured by mouse IL-4 ELISA kit (#431107, BioLegend). IL-5 and IL-33 were measured by Platinum ELISA (#BMS610, #BMS6025, eBioscience). Norepinephrine was measured by 2-CAT (A-N) Research ELISA (#BA-E5400, LDN). Circulating Leptin and Insulin were measured by Crystalchem INC and circulating NEFA was measured by FFA Quantification Colorimetric/Fluorometric Kit (#K612, Biovision). Triglycerides (GPO-PAP method), total cholesterol (CHOD-PAP method), high density lipoprotein cholesterol (direct method) and low-density lipoprotein cholesterol (direct method) in circulating were analyzed on the auto clinical chemistry analyzer platform (ZY-330, KHB). Blank Control and quality Controls were added in every measurement. All the processes were administrated according to the manufacturer's instructions.
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