Antibody against erk2 c14

Mouse monoclonal antibody directed against human MET (#3148) and rabbit polyclonal antibody directed against the phosphorylated tyrosine (Y 1234/1235) of MET (#3126) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal antibody against the kinase domain of MET (3D4) was purchased from Invitrogen. Mouse monoclonal antibody directed against the N-terminal domain of MET (DL-21) was kindly provided by Dr Sylvia Giordano (University of Torino Medical School, Italy) [50 (link)]. Mouse monoclonal antibody against lamin B2 (33–2100) was purchased from Zymed. Mouse monoclonal antibody against GAPDH (6C5-32233), rabbit polyclonal antibody against the C-terminal domain of human PARP-1 (H250), and antibody against ERK2 (C14) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Green-fluorescent Alexa fluor 488 conjugated anti-mouse IgG (H+L) and phalloidin– Alexa Fluor 546 were purchased from Invitrogen. Rabbit polyclonal antibodies against the Flag epitopes were purchased from Sigma (St Louis, MO, USA).

Compounds were used at the following concentrations unless otherwise noted: 50 μM AZD3988 (Tocris), 30 μM A922500 (Stratech), 50 μM AZD7687 (Stratech), 70 μM T863 (Sigma), 1 μM Oligomycin (Sigma), 0.5 μM FCCP (Sigma), 1 μM Antimycin-A (Sigma), 1 μM Rotenone (Sigma), 50 μM PF-06424439 (Sigma), 5 μM Ebselen (Tocris), 1 mM Tempol, 200 μM Paraquat (Sigma), Menadione (Sigma), 100 μM Etomoxir (Sigma), 2 μM Ferrostatin-1 (Sigma), 5 μM ML385 (Selleckchem), and 1 μM ATN-224 (Cayman Chemical). Antibodies against DGAT1 (ab54037) and 4-Hydroxynonenal (ab46545) were purchased from abcam. Antibodies against Vinculin (66305-1-Ig), Beta-Tubulin (10094-1-AP), PINK1 (23274-1-AP), Parkin (14060-1-AP), SOD1 (10269-1-AP), SOD2 (24127-1-AP), SESN2 (10795-1-AP) and GAPDH (60004-1-Ig) were purchased from Proteintech. Antibodies against phospho-AMPK (50081), phospho-RAPTOR (2083), Caspase-3 (9662), phospho-ERK (4370) and GFP (2956) were purchased from Cell Signalling. The antibody against gamma-tubulin (T5326) was purchased from Sigma. The antibody against ERK2 (C-14) was from Santa Cruz Biotechnology.