Palladium on carbon pd c

The synthesis of the dendrons N₃-dxDAP, where x represents the number of peripheral DAP units, is outlined in Scheme 2; Scheme 3, and the synthesis of thymine cross-linkers is outlined in Scheme 4. Compounds (1)-(5) were synthesized according to previously reported procedures [47 (link),48 (link),49 (link)]. Polyethylenglycol monomethyl ether (PEG_2k–OH) was purchased from Sigma Aldrich (Sigma Aldrich Gmbh, Steinheim, Germany) and used as received (according to certificate of analysis, batch 1257344: M_n = 1678 g mol⁻¹, Ð = 1.21; according to MALDI-TOF mass spectrometry: M_n = 1948.0 g mol⁻¹ corresponding to the more intense m/z peak) (Figure S1). Palladium on carbon (Pd/C) (10 wt% loading, matrix-activated carbon support) catalyst was purchased from Sigma Aldrich. Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) was purchased from ACROS Organics (Thermo Fisher Scientific, Geel, Belgium). The azide-functionalized Merrifield’s resin was prepared from Merrifield’s resin cross-linked with 1% divinylbenzene (200–400 mesh) (0.8–1.3 mmol g⁻¹) (TCI Europe N.V., Zwijndrecht, Belgium) according to the literature procedure [50 (link)]. Other reagents were purchased from Sigma Aldrich and used as received. Fourier Transformed Infrared Spectroscopy (FTIR) and 1H NMR spectra are collected at the Supporting Information (Figures S2–S35).

SMZ (>98%) was provided by China Institute of Veterinary Drug Control (Beijing, China). Palladium on Carbon (Pd/C) was obtained from Sigma-Aldrich (Shanghai, China). Reagents such as H₂O₂(30%), sodium chloride (Nacl) and anhydrous sodium sulfate (Na₂SO₄) were purchased from Sinopharm Chemical Reagent Co., LTD. (Shanghai, China). Solvable (tissue solubilizing fluid), and Ultima Gold scintillation fluid were obtained from PerkinElmer Life and Analytical Sciences (Groningen, The Netherlands; Waltham, MA, USA, respectively). Stop Flow AD scintillation liquid was purchased from AIM Research Co. (Hockessin, DE, USA). Water was purified by using a Milli-Q system (Bedford, MA, USA). All reagents and other chemicals are analytical grade or higher purity.