Blood was collected from the hearts of mice at the determined end points and centrifuged at 3000 rpm to isolate the plasma by a microvolume high-speed cooling centrifuge MX-201 (TOMY, Tokyo, Japan) and stored at −20 °C. The dorsal skin, livers, and kidneys were excised and homogenized with lysis buffer (Kurabo, Osaka, Japan), and then, the tissue lysate was centrifuged at 10,000 rpm. Plasma concentrations of interleukin (IL)-6, tumor necrosis factor (TNF)-α, creatinine, hyaluronic acid, endostatine, glutamate oxaloacetate transaminase (GOT), glutamic acid pyruvate transaminase (GPT), matrix metalloproteinase (MMP)-1, histamine, and angiopoitin 1 and 2 were measured using appropriate ELISA kits according to manufacturers’ instructions (IL-6, Enzo Life Sciences, Farmingdale, NY, USA; TNF-α, hyaluronic acid, and angiopoietin 2, R&D Systems, Minneapolis, MN, USA; MMP-1, MyBioSource, San Diego, CA, USA; histamine, Bertin Pharm, Montigny-le-Bretonneux, France; creatinine, Cayman, Ann Arbor, MI, USA; Endostatine, BioMedica, Vienna, Austria; angiopoietin 1, EIAAB Science, Wuhan, China; GOT and GPT, Wako, Osaka, Japan; ROS, Cell Biolabs Inc., San Diego, CA, USA). Optical density was measured using a microplate reader (Molecular Devices, Sunnyvale, CA, USA).
Creatinine
Manufactured by Enzo Life Sciences
Sourced in United States, Germany
Creatinine is a lab equipment product used for the measurement of creatinine levels in biological samples. Creatinine is a waste product formed from the breakdown of creatine, a compound found in muscle tissue. The measurement of creatinine levels is important for assessing kidney function.
Automatically generated - may contain errors
Lab products found in correlation
Hyaluronic acid, by R&D Systems (1 mentions)
Lysis buffer, by Kurabo (1 mentions)
Creatinine, by Cayman Chemical (1 mentions)
Interleukin 6 (il 6), by Enzo Life Sciences (1 mentions)
Mmp 1, by MyBioSource (1 mentions)
Mx 201, by TOMY (1 mentions)
Tumor necrosis factor (tnf), by Enzo Life Sciences (1 mentions)
Angiopoietin 2, by R&D Systems (1 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Tnf α, by R&D Systems (1 mentions)
2 protocols using creatinine
1
Plasma and Tissue Biomarker Analysis in Mice
2
Salivary and Urinary Biomarkers Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After rinsing their mouth with water thoroughly, participants collected saliva for over 3 min with a cotton plugged salivette. Salivettes were immediately kept in an icebox. After transportation to the laboratory, the tubes were centrifuged (2 min, 3000 rpm) and stored without preservatives at -20°C until analysis. Urine fractions were aliquoted in appropriate tubes and stored at -20°C until analysis.
The manufacturer's recommended protocols for saliva were used without modification for saliva cortisol, Immunoglobuline A (sIgA), C-reactive protein (CRP) (Salimetrics, State College, PA) and saliva alpha amylase (sAA) (IBL, Hamburg, Germany). For urine, protocols of neopterin (IBL, Hamburg, Germany), creatinine (Enzo Life Sciences, Framingdate, NY) and cortisol (Arbor assay, MI) were applied.
Analyses were performed on all 12-points for saliva collected for cortisol, IgA and sAA while sCRP analyses were performed only on two points: 10:00 and 16:30.
All samples were tested in duplicate, and the average of duplicates was used as the final value.
The manufacturer's recommended protocols for saliva were used without modification for saliva cortisol, Immunoglobuline A (sIgA), C-reactive protein (CRP) (Salimetrics, State College, PA) and saliva alpha amylase (sAA) (IBL, Hamburg, Germany). For urine, protocols of neopterin (IBL, Hamburg, Germany), creatinine (Enzo Life Sciences, Framingdate, NY) and cortisol (Arbor assay, MI) were applied.
Analyses were performed on all 12-points for saliva collected for cortisol, IgA and sAA while sCRP analyses were performed only on two points: 10:00 and 16:30.
All samples were tested in duplicate, and the average of duplicates was used as the final value.
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