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Osteocalcin rat elisa system

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The Osteocalcin rat ELISA system is a laboratory equipment used to measure the levels of osteocalcin, a protein found in bone, in rat samples. It is a quantitative enzyme-linked immunosorbent assay (ELISA) that utilizes specific antibodies to detect and quantify the presence of osteocalcin in the sample.

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Lab products found in correlation

Za3300, by Hitachi (2 mentions) Osteolinks dpd kit, by Sumitomo Pharma (2 mentions) Tba 120fr, by Toshiba (2 mentions) Mouse rat sost quantikine elisa kit, by R&D Systems (1 mentions) Calcium e ha test wako, by Fujifilm (1 mentions)

6 protocols using osteocalcin rat elisa system

1

Serum and Bone Mineral Analysis

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Serum zinc was measured using a metallo-assay kit (Metallogenics, Chiba, Japan). Serum iron was measured using the Fe C-test (Wako Pure Chem. Ind.). Serum magnesium was measured using the Mg B-test (Wako Pure Chem. Ind.). Minerals in other serum and bone samples were analyzed using atomic absorption spectrophotometry (ZA3300, Hitachi, Ltd., Tokyo, Japan) according to the method of Gimblet et al.(24 (link)) Bone phosphorus was analyzed colorimetrically according to the method of Gomori.(25 ) The serum osteocalcin concentration was measured using an Osteocalcin rat ELISA system (GE Healthcare UK Ltd., Amersham, England). The serum C-terminal telopeptide of type I collagen (CTx) level was assayed using the RatLaps ELISA (Immunodiagnostic systems A/S, Herlev, Denmark).
Suzuki T., Katsumata S.I., Matsuzaki H, & Suzuki K. (2015). Dietary zinc supplementation increased TNFα and IL1β-induced RANKL expression, resulting in a decrease in bone mineral density in rats. Journal of Clinical Biochemistry and Nutrition, 58(1), 48-55.
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2

Serum and Bone Mineral Analysis

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Serum zinc was measured using a metallo-assay kit (Metallogenics, Chiba, Japan). Serum calcium and zinc, and bone calcium and iron in femur were analyzed using atomic absorption spectrophotometry (ZA3300, Hitachi, Ltd., Tokyo, Japan) according to the method of Gimblet et al.(19 (link)) Bone phosphorus was analyzed colorimetrically according to the method of Gomori.(20 ) Serum Osteocalcin concentration was measured using an Osteocalcin Rat ELISA System (GE Healthcare UK Ltd., Amersham, England). The serum C-terminal telopeptide of type I collagen (CTx) level was assayed using the RatLaps ELISA (Immunodiagnostic Systems Nordic A/S, Herlev, Denmark).
Suzuki T., Katsumata S.I., Matsuzaki H, & Suzuki K. (2016). Dietary zinc deficiency induces oxidative stress and promotes tumor necrosis factor-α- and interleukin-1β-induced RANKL expression in rat bone. Journal of Clinical Biochemistry and Nutrition, 58(2), 122-129.
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3

Bone Metabolism Markers in Rat Serum

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The bone metabolic markers and biochemical data were measured in serum samples obtained from Groups 6, 7, and 8. The blood samplings were performed before the daily administration of saline or hPTH(1–34) on days 0 to 3. It was also performed at 6 h after the first administration of saline or hPTH(1–34) on day 0. The sampling on day 5 was performed at 24 h after the day 4 administration. All rats were fasted for at least 6 h before blood collection. Serum was obtained by centrifugation of the collected blood samples from the subclavian vein according to the time schedules as mentioned above. The serum samples were aliquoted and stored at − 80 °C until analysis.
The serum level of osteocalcin (OC) was determined using an osteocalcin rat ELISA system (GE Healthcare, Piscataway, NJ, USA). The serum level of sclerostin (SOST) was measured using Mouse/Rat SOST Quantikine ELISA Kit (R&D Systems, Minneapolis, MN). The following serum biochemical assays were performed: inorganic phosphorus (Pi) using L-type Wako Pi (Wako Pure Chemical Industries, Osaka, Japan), calcium (Ca) using Calcium E-HA test Wako (Wako Pure Chemical Industries), alkaline phosphatase (ALP) activity using L-type Wako ALP ∙ J (Wako Pure Chemical Industries). All assays were performed according to the manufacturer's instructions.
Ogura K., Iimura T., Makino Y., Sugie-Oya A., Takakura A., Takao-Kawabata R., Ishizuya T., Moriyama K, & Yamaguchi A. (2016). Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone. Bone Reports, 5, 7-14.
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4

Measurement of bone turnover markers

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At the completion of the 3-month administration period, blood was collected before and 6, 24, 72, and 144 hr after the final dose for the once-weekly group, and before and 6 and 24 hr after the final dose for the once-daily group. ELISA was performed to measure serum levels of osteocalcin (OC) and cross-linked N-telopeptide of type 1 collagen (NTx) using an osteocalcin rat ELISA system (GE Healthcare Japan Corp., Tokyo, Japan) and OSTEOMARK NTx Serum (Alere Medical Co., Ltd., Tokyo, Japan), respectively, and the OC and NTx concentrations were determined based on the calibration curves constructed using their respective reference standards. Means of duplicates were used as the measured concentrations.
Watanabe A., Tsurui K., Yoneyama S., Iwata H., Anzai T., Jerome C, & Nakae D. (2018). Effect of dosing frequency of teriparatide (PTH 1-34) on bone formation in rats: comparison of bone metabolism marker levels. The Journal of toxicological sciences, 43(7).
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5

Bone Metabolism Markers Measurement

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Glycosylated hemoglobin A1c (HbA1c), serum calcium (Ca), urinary Ca, and urinary creatinine (Cr) were measured with an autoanalyzer (TBA-120FR; Toshiba Medical Systems Co., Tochigi, Japan). Urinary deoxypyridinoline (DPD) was measured as a bone resorption marker using an Osteolinks-DPD kit (DS Pharma Biomedical, Osaka, Japan) and the data were corrected for urinary Cr concentration. Serum osteocalcin (OC) was measured as a bone formation marker using an Osteocalcin Rat ELISA System (GE Healthcare Japan Co., Tokyo, Japan).
Takeda S., Saito M., Sakai S., Yogo K., Marumo K, & Endo K. (2017). Eldecalcitol, an Active Vitamin D3 Derivative, Prevents Trabecular Bone Loss and Bone Fragility in Type I Diabetic Model Rats. Calcified Tissue International, 101(4), 433-444.
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6

Urinary DPD and Serum Osteocalcin Analysis

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Urinary deoxypyridinoline (DPD) was measured using an Osteolinks-DPD kit (DS Pharma Biomedical, Osaka, Japan) and the data were corrected for urinary creatinine (Cr) concentration. Cr was measured with an autoanalyzer (TBA-120FR; Toshiba Medical Systems Co., Tochigi, Japan). Serum osteocalcin (OCN) was measured using an Osteocalcin Rat ELISA System (GE Healthcare Japan Co., Tokyo, Japan).
Takeda S., Sakai S., Tanaka K., Tomizawa H., Serizawa K., Yogo K., Urayama K., Hashimoto J., Endo K, & Matsumoto Y. (2017). Intermittent Ibandronate Maintains Bone Mass, Bone Structure, and Biomechanical Strength of Trabecular and Cortical Bone After Discontinuation of Parathyroid Hormone Treatment in Ovariectomized Rats. Calcified Tissue International, 101(1), 65-74.
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