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Shrna vector

Manufactured by OriGene
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The ShRNA vector is a plasmid-based system designed for the expression of short hairpin RNA (shRNA) in mammalian cells. The vector contains the necessary elements for the transcription and processing of shRNA, which can be used to knockdown the expression of target genes.

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Lab products found in correlation

Stbl3 bacteria, by Thermo Fisher Scientific (2 mentions) Clonejet kit, by Thermo Fisher Scientific (2 mentions) Pdonr223 pi4kap2, by Addgene (2 mentions) Mnd gfp cassette, by OriGene (2 mentions)

Close spelling variants of this product

Human Elmo1 targeting or control shRNA pGFP-V-RS expression vectors HuSH 29-mer shRNA expression vectors PGFP-C-shLenti shRNA vector PGFP-V-RS shRNA Vector PRS shRNA vector HuSH shRNA GFP Lenti Cloning Vector ALDH1B1 shRNA in pRFP-C-RS plasmid vector

2 protocols using shrna vector

1

Cloning and Ligation of Pi4kap2 into pLV Vector

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The Pi4kap2 coding sequence was amplified from pDONR223-PI4KAP2 (23601 Addgene) using primers (Supplemental Experimental Procedures) that introduced an HA-tag at the 3′ end and flanked the fragment with PstI and AgeI restriction enzyme sequences. The fragment was ligated into the pJet cloning vector using CloneJET kit instructions (K1231 Thermo Fisher Scientific) and amplified in Stbl3 bacteria (C737303 Thermo Fisher Scientific). The pLV-Ef1a-MCS-IRES-RFP-puro plasmid and the Pi4kap2HA-pJet vector were linearized with PstI and AgeI. The Pi4kap2-HA fragment was gel purified and ligated into the pLV vector using T4 ligase. For the shRNA vector purchased from Origene (TL510615), the MND-GFP cassette (a gift from the Kohn Laboratory) was used to replace the CMV-GFP reporter.
Ziyad S., Riordan J.D., Cavanaugh A.M., Su T., Hernandez G.E., Hilfenhaus G., Morselli M., Huynh K., Wang K., Chen J.N., Dupuy A.J, & Iruela-Arispe M.L. (2018). A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis. Cell reports, 22(5), 1211-1224.
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2

Cloning and Ligation of Pi4kap2 into pLV Vector

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Pi4kap2 coding sequence was amplified from pDONR223-PI4KAP2 (23601 Addgene) using primers (Supplemental Experimental Procedures) that introduced an HA-tag at the 3′ end and flanked the fragment with PstI and AgeI restriction enzyme sequences. The fragment was ligated into the pJet cloning vector using CloneJET kit instructions (K1231 Thermo Fisher Scientific) and amplified in Stbl3 bacteria (C737303 Thermo Fisher Scientific). The pLV-Ef1a-MCS-IRES-RFP-puro plasmid and the Pi4kap2HA-pJet vector were linearized with PstI and AgeI. The Pi4kap2-HA fragment was gel purified and ligated into the pLV vector using T4 ligase. For the shRNA vector purchased from Origene (TL510615), the MND-GFP cassette (a gift from the Kohn Laboratory) was used to replace the CMV-GFP reporter.
Ziyad S., Riordan J.D., Cavanaugh A.M., Su T., Hernandez G.E., Hilfenhaus G., Morselli M., Huynh K., Wang K., Chen J.N., Dupuy A.J, & Iruela-Arispe M.L. (2018). A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis. Cell reports, 22(5), 1211-1224.
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