Mouse anti human nuclear antigen hna

Manufactured by Merck Group

The Mouse anti-human nuclear antigen (HNA) is a laboratory reagent used to detect the presence of a specific nuclear antigen in human cells. It is a monoclonal antibody that recognizes and binds to the target antigen, allowing for its identification and analysis in various research and diagnostic applications.

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Lab products found in correlation

Rabbit anti e cadherin, by Cell Signaling Technology (2 mentions) Alexa 633 phalloidin, by Thermo Fisher Scientific (2 mentions) Mouse anti skeletal myosin, by Merck Group (2 mentions) Rabbit anti β1, by Abcepta (2 mentions) Mouse anti fyn, by BioLegend (2 mentions) Mouse anti cd44, by Bio-Rad (2 mentions) Alexa 568 conjugated goat anti mouse rabbit, by Thermo Fisher Scientific (2 mentions) Eclipse te200 fluorescent microscope, by Zeiss (2 mentions) Axio observer z1 microscope, by Zeiss (2 mentions) Rabbit anti activated caspase 3, by R&D Systems (2 mentions) Rabbit anti cd31, by Santa Cruz Biotechnology (2 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (2 mentions) Rabbit anti ki67, by Abcam (2 mentions) Protein block solution, by Agilent Technologies (1 mentions) Saponin, by Merck Group (1 mentions) Axio scan z1, by Zeiss (1 mentions)

Close spelling variants of this product

Anti-human nuclear antigen (HNA; Mouse anti-human nuclear antigen Human nuclear antigen Mouse anti-HNA

3 protocols using mouse anti human nuclear antigen hna

Cardiomyocyte Differentiation and Heart Histology

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CDCs were cultured in cardiomyocyte differentiation media (Millipore, Billerica, MA), after which the cells were fixed with 4% PFA, blocked/permeabilized with protein block solution (Dako) containing 1% saponin (Sigma‐Aldrich), and then stained with chicken anti‐CD105 (Sigma‐Aldrich) and mouse anti‐αSA (Sigma‐Aldrich). FITC or Texas Red secondary Abs were obtained from Abcam. For heart histology, all animals were sacrificed at 3 weeks (after echocardiography study) and excised hearts were cryosectioned (5‐μm thickness). Heart cryosections were then fixed with 4% PFA, blocked/permeabilized, and stained with mouse anti‐human nuclear antigen (HNA; Millipore) and rabbit anti‐αSA (Abcam). Images were taken with a confocal microscope. Detailed Ab information is included in Table S1.

Cheng K., Ibrahim A., Hensley M.T., Shen D., Sun B., Middleton R., Liu W., Smith R.R, & Marbán E. (2014). Relative Roles of CD90 and c‐Kit to the Regenerative Efficacy of Cardiosphere‐Derived Cells in Humans and in a Mouse Model of Myocardial Infarction. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(5), e001260.

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Immunohistochemical Analysis of Tissue Samples

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For H&E staining, sections were stained with Gill's hematoxylin and eosin Y and then scanned at 20X using a Zeiss AxioScan.Z1 slide scanner. The following primary antibodies were used for IHC/ICC¹⁵: rabbit anti-Ki67 (1:5,000; Abcam); rabbit anti-activated caspase-3 (1:200; R&D Systems); rabbit anti-CD31 (Santa Cruz Biotechnology); mouse anti-skeletal myosin (1:400; Sigma); rabbit anti-β1 (1:200; Abgent); mouse anti-fyn (1:100; BioLegend); mouse anti-CD44 (1:100; AbD Serotec); rabbit anti-E-cadherin (1:200; Cell Signaling Technology); mouse anti-human nuclear antigen (HNA; 1:100; Millipore). Secondary antibodies were Alexa-568-conjugated goat anti mouse/rabbit, unless stated otherwise (1:500; Invitrogen). Samples were mounted in Prolong Gold with DAPI (Invitrogen). Some samples were stained with Alexa-633-phalloidin (1:25; Invitrogen).23 (link) Samples were viewed using 20× objectives on a Nikon Eclipse TE200 fluorescent microscope, or Zeiss Axio Observer.Z1 microscope with LSM 710 confocal laser scanner.

Nelson M., Millican-Slater R., Forrest L.C, & Brackenbury W.J. (2014). The sodium channel β1 subunit mediates outgrowth of neurite-like processes on breast cancer cells and promotes tumour growth and metastasis. International Journal of Cancer. Journal International du Cancer, 135(10), 2338-2351.

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Immunostaining and Microscopy Protocol

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For H&E staining, sections were stained with Gill’s hematoxylin and eosin Y and then scanned at 20× using a Zeiss AxioScan.Z1 slide scanner. The following primary antibodies were used for IHC/ICC^{15 (link)}: rabbit anti-Ki67 (1:5,000; Abcam); rabbit anti-activated caspase-3 (1:200; R&D Systems); rabbit anti-CD31 (Santa Cruz Biotechnology); mouse anti-skeletal myosin (1:400; Sigma); rabbit anti-β1 (1:200; Abgent); mouse anti-fyn (1:100; BioLegend); mouse anti-CD44 (1:100; AbD Serotec); rabbit anti-E-cadherin (1:200; Cell Signaling Technology); mouse anti-human nuclear antigen (HNA; 1:100; Millipore). Secondary antibodies were Alexa-568-conjugated goat anti mouse/rabbit, unless stated otherwise (1:500; Invitrogen). Samples were mounted in Prolong Gold with DAPI (Invitrogen). Some samples were stained with Alexa-633-phalloidin (1:25; Invitrogen).^{23 (link)} Samples were viewed using 20× objectives on a Nikon Eclipse TE200 fluorescent microscope, or Zeiss Axio Observer.Z1 microscope with LSM 710 confocal laser scanner.

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