Samples are library prepped depending on whether an R9 or R10 flowcell is to be used. For R9 flowcells, the Oxford Nanopore RBK004 kit was used, using 600 ng input material and following manufacturer’s instructions for other steps. For R10 flowcells, we used the Oxford Nanopore technologies RBK114-24 kit, but with an adjusted protocol: for optimal results (size distribution centred around 5 kb) 3,500 ng of input material in 50 μl is first sheared using a G-Tube (Covaris SKU: 520079), centrifuging at 7,200 RPM (6,000g) in a fixed rotor tabletop centrifuge. Subsequently 7.5 μl of the sheared input material is used for tagmentation with 2.5 μl indexing mix. Alternatively, we obtain similar results (but with wider size distributions) using 200 ng input material for the tagmentation without an added fragmentation step. For both protocols, we omit the AMPure purification, and after tagmentation directly proceed with adapter ligation.
Rbk114 24 kit
Manufactured by Oxford Nanopore
The RBK114-24 kit is a laboratory equipment product offered by Oxford Nanopore. It is a starter kit for DNA/RNA sequencing using nanopore technology. The kit contains the necessary components to perform library preparation and run sequencing experiments. However, a detailed description of the kit's core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rbk114 24 kit
1
Nanopore Library Prep for R9 and R10
2
Nanopore Library Prep for R9 and R10
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples are library prepped depending on whether an R9 or R10 flowcell is to be used. For R9 flowcells, the Oxford Nanopore RBK004 kit was used, using 600 ng input material and following manufacturer’s instructions for other steps. For R10 flowcells, we used the Oxford Nanopore technologies RBK114-24 kit, but with an adjusted protocol: for optimal results (size distribution centred around 5 kb) 3,500 ng of input material in 50 μl is first sheared using a G-Tube (Covaris SKU: 520079), centrifuging at 7,200 RPM (6,000g) in a fixed rotor tabletop centrifuge. Subsequently 7.5 μl of the sheared input material is used for tagmentation with 2.5 μl indexing mix. Alternatively, we obtain similar results (but with wider size distributions) using 200 ng input material for the tagmentation without an added fragmentation step. For both protocols, we omit the AMPure purification, and after tagmentation directly proceed with adapter ligation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!