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Human recombinant or bovine insulin

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Human recombinant or bovine insulin is a laboratory product used for research and development purposes. It serves as a core component in the study and analysis of insulin-related biochemical processes. The product provides a standardized source of insulin for scientific investigations, without interpretation or extrapolation on its intended use.

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Lab products found in correlation

Fetal bovine serum (fbs), by Merck Group (2 mentions) Recombinant human albumin, by Merck Group (2 mentions)

Spelling variants of this product

Insulin (4936 citations) Bovine insulin (422 citations) Human insulin (402 citations) Recombinant human insulin (252 citations) Recombinant insulin (13 citations) Recombinant bovine insulin (2 citations)

2 protocols using human recombinant or bovine insulin

1

Cardiac Progenitor Cell Culture Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
At day 6, cardiac progenitor cells were singularized with Accutase at 37°C for 5 min and then seeded onto a gelatin- or SyntheMax-coated cell culture plate at 20,000–80,000 cells/cm2 (or 1:3–1:12 split) in LaSR basal medium (advanced DMEM/F12 with 100 μg/mL ascorbic acid) or RPMI/Vc/Ins medium (100 μg/mL ascorbic acid and 1 μg/mL human recombinant or bovine insulin (Sigma)) with 5 μM ROCK inhibitor Y-27632 for 24 hours. The addition of 1% human recombinant albumin (Sigma) or fetal bovine serum (FBS) can improve cell attachment and survival, but they are not required. At day 7, cells were treated with 1–9 μM CHIR99021 for 2 days in LaSR basal medium or RPMI/insulin/Vc medium. CHIR99021-containing medium was aspirated on day 9 and cells were cultured in LaSR basal medium or RPMI/insulin/Vc medium without CHIR99021 for 3–5 additional days, as explained previously14 (link).
Bao X., Lian X., Hacker T.A., Schmuck E.G., Qian T., Bhute V.J., Han T., Shi M., Drowley L., Plowright A., Wang Q.D., Goumans M.J, & Palecek S.P. (2016). Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions. Nature biomedical engineering, 1, 0003.
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2

Cardiac Progenitor Cell Culture Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
At day 6, cardiac progenitor cells were singularized with Accutase at 37°C for 5 min and then seeded onto a gelatin- or SyntheMax-coated cell culture plate at 20,000–80,000 cells/cm2 (or 1:3–1:12 split) in LaSR basal medium (advanced DMEM/F12 with 100 μg/mL ascorbic acid) or RPMI/Vc/Ins medium (100 μg/mL ascorbic acid and 1 μg/mL human recombinant or bovine insulin (Sigma)) with 5 μM ROCK inhibitor Y-27632 for 24 hours. The addition of 1% human recombinant albumin (Sigma) or fetal bovine serum (FBS) can improve cell attachment and survival, but they are not required. At day 7, cells were treated with 1–9 μM CHIR99021 for 2 days in LaSR basal medium or RPMI/insulin/Vc medium. CHIR99021-containing medium was aspirated on day 9 and cells were cultured in LaSR basal medium or RPMI/insulin/Vc medium without CHIR99021 for 3–5 additional days, as explained previously14 (link).
Bao X., Lian X., Hacker T.A., Schmuck E.G., Qian T., Bhute V.J., Han T., Shi M., Drowley L., Plowright A., Wang Q.D., Goumans M.J, & Palecek S.P. (2016). Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions. Nature biomedical engineering, 1, 0003.
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