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Cvivo15 x vivo 15 media

Manufactured by Lonza
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CVIVO15 is a serum-free and chemically defined cell culture medium developed by Lonza. It is formulated to support the growth and expansion of a variety of cell types, including primary cells, stem cells, and immortalized cell lines. The medium is designed to provide a consistent and reliable environment for cell culture applications.

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Lab products found in correlation

Rlt buffer, by Qiagen (2 mentions) Rneasy extraction kit, by Qiagen (2 mentions) Fetal bovine serum (fbs), by Gemini Bio (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) N acetyl l cysteine, by Merck Group (2 mentions) Novaseq, by Illumina (2 mentions)

Spelling variants of this product

X-VIVO 15 (360 citations) X-VIVO 15 media (143 citations) X-VIVO (56 citations) X-Vivo media (37 citations)

2 protocols using cvivo15 x vivo 15 media

1

PBMC Thawing and RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were thawed in warm cVIVO15 (X-VIVO™ 15 Media (Lonza) supplemented with 5% FBS (Gemini Bio), 55 uM 2-mercaptoethanol (Sigma), and 10 mM N-acetyl-L cysteine (Sigma)). We washed cells twice with cVIVO15, resuspended in PBS and spun down. Cell pellets were resuspended in RLT+ buffer (Qiagen) and flash frozen on dry ice. For RNA isolation, we thawed, vortexed, and incubated samples for 5 minutes at room temperature before RNA isolation using the Qiagen RNeasy extraction kit. After isolation, libraries were generated at the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute and sequenced on an Illumina NovaSeq.
Nathan A., Beynor J.I., Baglaenko Y., Suliman S., Ishigaki K., Asgari S., Huang C.C., Luo Y., Zhang Z., Lopez K., Arlehamn C.S., Ernst J.D., Jimenez J., Calderón R.I., Lecca L., Van Rhijn I., Moody D.B., Murray M.B, & Raychaudhuri S. (2021). Multimodally profiling memory T cells from a tuberculosis cohort identifies cell state associations with demographics, environment, and disease. Nature immunology, 22(6), 781-793.
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2

PBMC Thawing and RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were thawed in warm cVIVO15 (X-VIVO™ 15 Media (Lonza) supplemented with 5% FBS (Gemini Bio), 55 uM 2-mercaptoethanol (Sigma), and 10 mM N-acetyl-L cysteine (Sigma)). We washed cells twice with cVIVO15, resuspended in PBS and spun down. Cell pellets were resuspended in RLT+ buffer (Qiagen) and flash frozen on dry ice. For RNA isolation, we thawed, vortexed, and incubated samples for 5 minutes at room temperature before RNA isolation using the Qiagen RNeasy extraction kit. After isolation, libraries were generated at the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute and sequenced on an Illumina NovaSeq.
Nathan A., Beynor J.I., Baglaenko Y., Suliman S., Ishigaki K., Asgari S., Huang C.C., Luo Y., Zhang Z., Lopez K., Arlehamn C.S., Ernst J.D., Jimenez J., Calderón R.I., Lecca L., Van Rhijn I., Moody D.B., Murray M.B, & Raychaudhuri S. (2021). Multimodally profiling memory T cells from a tuberculosis cohort identifies cell state associations with demographics, environment, and disease. Nature immunology, 22(6), 781-793.
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