Ab89621

Manufactured by Abcam

Sourced in United Kingdom

Ab89621 is a lab equipment product offered by Abcam. It serves as a core function to facilitate specific laboratory processes.

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Lab products found in correlation

2 protocols using ab89621

Comprehensive Immunoblotting Assay Protocol

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Immunoblotting assays were carried out as previously described⁹ with the following primary Abs: DAB2IP (23582‐1‐AP, Proteintech), c‐Myc (ab32072, Abcam), c‐Myc (67447‐1‐Ig, Proteintech), p‐c‐Myc^S62 (ab185656, Abcam), p‐c‐Myc^T58 (ab185655, Abcam), glycogen synthase kinase 3β (GSK3β; 10044‐T32, SinoBiological), p‐GSK3β^S9 (#5558, CST), Bcl‐2 (12789‐1‐AP, Proteintech), Bax (#5023, CST), B56α (ab89621, Abcam), Ubiquitin (10201‐2‐AP, Proteintech), Ubiquitin (linkage‐specific K48) (ab140601, Abcam), Nanog (#8822S, CST), CD44 (60224‐1‐Ig, Proteintech), CD133 (18470‐1‐AP, Proteintech), and GAPDH (60004‐1‐Ig, Proteintech). Secondary Abs conjugated to HRP (Servicebio) were used, followed by chemiluminescence.

Li H., Zhou Y., Wang M., Wang H., Zhang Y., Peng R., Zhang R., Zhang M., Zhang M., Qiu P., Liu L., Zhao Q, & Liu J. (2021). DOC‐2/DAB2 interactive protein destabilizes c‐Myc to impair the growth and self‐renewal of colon tumor‐repopulating cells. Cancer Science, 112(11), 4593-4603.

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Antibody Characterization for Immunoblotting, Flow Cytometry, and Immunoprecipitation

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The following primary antibodies were used for immunoblot (alphabetical order): anti-APOBEC3G (AIDS Reagent Program, Division of AIDS, NIAID, NIH from Immunodiagnostics, 10069), anti-ß-catenin (ab32572, Abcam, UK), anti-calreticulin (PA3-900, Thermo Scientific), anti-FLAG (M2, Sigma), anti-HA (16B12, Biolegend), anti-p24 (ab9071, Abcam), anti-PPP2R5A (ab89621, Abcam), anti-PPP2R5D (ab88075, Abcam), anti-PPP2R5D (EPR15617, ab188323, Abcam), anti-RRM2 (ab57653, Abcam), anti-Vif (#319, AIDS Reagent Program, Division of AIDS, NIAID, NIH: Dr. Michael Malim #6459 (Fouchier et al., 1996 (link); Simon et al., 1997 (link)).
The following primary antibodies were used for flow cytometry: anti-CD4-AF647 (clone OKT4; BioLegend, UK), anti-HA-DyLight 650 (16B12, ab117515, Abcam), anti-PPP2R5D (EPR15617, ab188323, Abcam), anti-ICAM3 (TU41, 555957, BD).
The following primary antibodies were used for immunoprecipitation anti-PPP2R5D (EPR15617, ab188323, Abcam).
The following secondary antibodies were used: goat anti-mouse-AF647 and donkey anti-rabbit-AF647 (flow cytometry, Molecular Probes, Thermo Fisher Scientific); goat anti-mouse-HRP and anti-rabbit-HRP (immunoblot, Jackson ImmunoResearch, West Grove, PA).

Greenwood E.J., Matheson N.J., Wals K., van den Boomen D.J., Antrobus R., Williamson J.C, & Lehner P.J. (2016). Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. eLife, 5, e18296.

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