Anti par 4

The following reagents were used: Doxorubicin (Sigma), Etoposide (Sigma), Z-IETD-FMK (Santa Cruz), LCL161 (Selleckchem), anti-PAR-4 (sc-1807, Santa Cruz), anti-PAR-4 (ab5787, Abcam), anti-GAPDH (sc-32233, Santa Cruz), anti-caspase-8 (#9746, Cell Signaling), anti-cleaved caspase-8 (#9496, Cell Signaling), anti-PARP-1 (#9542, Cell Signaling), anti-cIAP1 (#7065, Cell Signaling), anti-cIAP2 (#3130, Cell Signaling), anti-XIAP (#2045, Cell Signaling), anti-phospho-p65 Ser536 (#3033, Cell Signaling), anti-IκBα (#9242, Cell Signaling), anti-GFP (Rockland), anti-rabbit-HRP (P0448, DAKO), anti-mouse-HRP (P0447, DAKO), anti-rabbit-Alexa-Fluor-555 (A-31572, Thermo Fisher) and anti-rabbit-Alexa-Fluor-488 (A-21206, Thermo Fisher).

PAR-4 was detected via three different polyclonal antibodies. The C-terminus of PAR-4 was detected by using the anti-PAR-4 antibodies (Cell Signaling, #2328) or anti-PAR-4 (Abcam, #49155), while the N-terminal part was detected using anti-PAR-4 antibodies (Santa Cruz, #1807). Additional antibodies that were used are anti-PARP-1/ARTD-1 (Cell Signaling, #9542), anti-GAPDH 6C5 (Santa Cruz, #32233), anti-HA Y11 (Santa Cruz, #805), anti-caspase-3 (Cell Signaling, #9662) and anti-T7 (Novagen, #69522). Secondary antibodies used were horseradish peroxidase-labeled IgG goat anti-rabbit (#P0448) and rabbit anti-mouse (#P0161) from Dako. For confocal microscopy secondary goat anti-rabbit IgG conjugated with Alexa Fluor® 555 dye (Invitrogen, #A31572) was used.