Ml221

Manufactured by Bio-Techne

The ML221 is a laboratory tool designed for automated liquid handling. It features precise pipetting and mixing capabilities to support various applications in life science research and development.

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Lab products found in correlation

2 protocols using ml221

Comprehensive Protein Analysis Protocol

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Phosphate buffered saline (PBS), DMEM/F12 medium and trypsin were purchased from Gibco; Thermo Fisher Scientific, Inc. Insulin, glycerol, EDTA, dithiothreitol, 3,3′-diaminobenzidine (DAB), bromophenol blue, sodium deoxycholate, Nonidet P-40 (NP-40), Tween-20, PD098059, AG490 and apelin-13 (cat. no. A6469) were obtained from Sigma-Aldrich; Merck KGaA. Foetal bovine serum (FBS; heat inactivated) was purchased from Biowest. Tris base, SDS and bovine serum albumin (BSA) were purchased from Bioshop (Canada, Inc.). ML221, LY294002 and Compound C were obtained from Tocris Bioscience, Cell Signaling Technology, Inc. and Merck KGaA, respectively. The WesternBright™ Sirius kit was purchased from Advansta, Inc. Bradford protein assay kit, 4-20% gels (cat. no. 456-1093) and membranes (cat. no. 1704156) were obtained from Bio-Rad Laboratories, Inc.

Mlyczyńska E., Kurowska P., Drwal E., Opydo-Chanek M., Tworzydło W., Kotula-Balak M, & Rak A. (2020). Apelin and apelin receptor in human placenta: Expression, signalling pathway and regulation of trophoblast JEG-3 and BeWo cells proliferation and cell cycle. International Journal of Molecular Medicine, 45(3), 691-702.

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Apelin Pathway Modulation in Lung Repair

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The role of the apelin pathway in lung microvascular repair was assessed using a selective apelin receptor antagonist, ML221 (Tocris, 10 mg/kg) (Maloney et al., 2012 (link)) dissolved in DMSO delivered by intraperitoneal injection as previously described (Ishimaru et al., 2017 (link)).

Godoy R.S., Cober N.D., Cook D.P., McCourt E., Deng Y., Wang L., Schlosser K., Rowe K, & Stewart D.J. (2023). Single-cell transcriptomic atlas of lung microvascular regeneration after targeted endothelial cell ablation. eLife, 12, e80900.

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