The cell lines used in this study: HaCaT, immortalized human keratinocytes (cat. no. 300493; CLS Cell Lines Service GmbH, Eppelheim, Germany), 1BR3, human skin fibroblasts (cat. no. 90011801; European Collection of Authenticated Cell Cultures, Salisbury, UK), A375, human melanoma cells (ATCC® CRL-1619™), and B16 melanoma 4A5 cell line from mouse (ECACC; cat. no. 94042254) were acquired as frozen items and stored in liquid nitrogen until the experiment began. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit was purchased from Roche Diagnostics GmbH (Mannheim, Germany). The reagents used for cell culture: Dulbecco's modified Eagle's medium (DMEM) was provided from ATTC and trypsin/EDTA solution, phosphate-buffered saline (PBS), penicillin/streptomycin mixture, fetal calf serum (FCS) and Trypan blue solution were supplied by Sigma-Aldrich; Merck KGaA.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt kit
Manufactured by Roche
Sourced in Germany
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit is a colorimetric assay used to measure cell metabolic activity. The kit contains the MTT reagent, which is reduced by living cells to form a purple formazan product, and a solubilization solution to dissolve the formazan crystals. The absorbance of the resulting solution is proportional to the number of viable cells present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Atcc crl 1619, by American Type Culture Collection (1 mentions)
Penicillin streptomycin mixture, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Trypan blue solution, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
2 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt kit
1
Cell Line Characterization for Research
2
Olive Leaves Extraction and Cytotoxicity
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Solvents, methanol and ethanol, acetic acid, ammonium acetate were acquired from Merck (Darmstadt, Germany) while standard polyphenolic compounds and standard pentacyclic triterpenes were purchased from Sigma-Aldrich (Germany), 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) kit from Roche (Germany), specific cell culture reagents (medium, antibiotic mixture, foetal bovine serum, etc.) from American Type Culture Collection (ATCC). Olive leaves were collected from Ksamil (Southern Albania), certified at the Pharmaceutical Botany Department, Faculty of Pharmacy, Timișoara, Romania, and then dried (at room temperature, away from light, for 10 days) and ground.
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