For proliferation assays cells were plated in 384-well plates at a density of 800 cells per well in 40 µl total volume. One day later, a serial dilution of each inhibitor was performed using a D300e dispenser (Tecan). 96 h post-treatment, cell viability was determined using CellTiterGlo reagent (Promega) and luminescence quantified on an Envision MultiLabel Plate Reader (PerkinElmer). To calculate the fraction cell viability drug-treated cells were normalized to average cell viability of DMSO-only treated cells. Curve fitting was performed using GraphPad Prism v9.1.1 four-parameter inhibitor response with variable slope. AUC values were calculated by GraphPad Prism v9.1.1. Inhibitors were obtained from SelleckChem: Erlotinib-OSI-744 (S1023), Osimertinib-AZD9291 (S7297), Torin 1 (S2827), Alisertib-MLN8237 (S1133), Barasertib-AZD1152 (S1147). DMSO (Sigma-Aldrich).
Barasertib azd1152
Manufactured by Selleck Chemicals
Barasertib-AZD1152 is a small molecule inhibitor that selectively targets the Aurora B kinase. It is used as a research tool in laboratory settings to study the role of Aurora B kinase in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Alisertib mln8237, by Selleck Chemicals (2 mentions)
Envision multilabel plate reader, by PerkinElmer (2 mentions)
Torin1, by Selleck Chemicals (2 mentions)
Osimertinib azd9291, by Selleck Chemicals (2 mentions)
D300e dispenser, by Tecan (2 mentions)
Erlotinib osi 744, by Selleck Chemicals (2 mentions)
Celltiter glo reagent, by Promega (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
2 protocols using barasertib azd1152
1
Cell Viability Assay for Inhibitor Screening
2
Proliferation Assay with Kinase Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For proliferation assays cells were plated in 384-well plates at a density of 800 cells per well in 40 µl total volume. One day later, a serial dilution of each inhibitor was performed using a D300e dispenser (Tecan). 96 hours post-treatment, cell viability was determined using CellTiterGlo reagent (Promega) and luminescence quantified on an Envision MultiLabel Plate Reader (PerkinElmer). To calculate the fraction cell viability drug-treated cells were normalized to average cell viability of DMSO-only treated cells. Curve fitting was performed using GraphPad Prism four parameter inhibitor response with variable slope. AUC values were calculated by GraphPad Prism (Graphpad). Inhibitors were obtained from SelleckChem: Erlotinib-OSI-744 (S1023), Osimertinib-AZD9291 (S7297), Torin 1 (S2827), Alisertib-MLN8237 (S1133), Barasertib-AZD1152 (S1147). DMSO (Sigma-Aldrich).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!