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Alexa fluro 488 conjugated anti mouse antibodies

Manufactured by Thermo Fisher Scientific
Sourced in United States

Alexa Fluor 488-conjugated anti-mouse antibodies are fluorescent-labeled secondary antibodies used for detection and visualization in various immunoassay techniques. They are designed to bind to mouse primary antibodies, allowing for specific and sensitive detection of target antigens.

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2 protocols using alexa fluro 488 conjugated anti mouse antibodies

1

Immunofluorescence Staining for CD82 and Phospho-c-Met

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The immunofluorescence staining was carried out as previously described87 (link). In brief, cells were cultured on glass bottom cell culture dishes, fixed and washed. Mouse MAb to CD82 (Abcam, Cambridge, MA, USA) and rabbit PAb to phospho-c-Met (Y1356) were labeled with secondary Alexa Fluro 555-conjugated anti-rabbit and Alexa Fluro 488-conjugated anti-mouse antibodies (Invitrogen), respectively. The cells were counterstained with DAPI. Images were observed and recorded with a Zeiss Axiovert 200M epifluorescence microscope (Carl Zeiss Inc., Freistaat Thuüringen, Germany).
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2

Immunofluorescence Staining for CD82 and Phospho-c-Met

Check if the same lab product or an alternative is used in the 5 most similar protocols
The immunofluorescence staining was carried out as previously described87 (link). In brief, cells were cultured on glass bottom cell culture dishes, fixed and washed. Mouse MAb to CD82 (Abcam, Cambridge, MA, USA) and rabbit PAb to phospho-c-Met (Y1356) were labeled with secondary Alexa Fluro 555-conjugated anti-rabbit and Alexa Fluro 488-conjugated anti-mouse antibodies (Invitrogen), respectively. The cells were counterstained with DAPI. Images were observed and recorded with a Zeiss Axiovert 200M epifluorescence microscope (Carl Zeiss Inc., Freistaat Thuüringen, Germany).
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