Bckdk e 12

The BCKDK (E-12) (1:1000, sc-374425) antibodies were purchased from Santa Cruz Technology. Flag tag and talin-1 (#4021) antibodies were purchased from Sigma-Aldrich. All the following antibodies were purchased from Cell Signaling Technology: ERK1/2 (#4695), p-ERK1/2 (Thr202/Tyr204) (#4649), MEK1/2 (8727s), p-MEK1/2 (S221) (2338s), β1-integrin (D6S1W), TRIM21 (#92043), AKT (#4060), p-AKT (S473) (#13038), HA tag antibody (#3724), β-actin mouse monoclonal antibody (#3700), Anti-rabbit IgG (Alexa Fluor#488 Conjugate) (4412), Anti-mouse IgG (Alexa Fluor#594 Conjugate) (8890), secondary antibody against mouse and rabbit. The FAK (A11131), pY397-FAK (AP0302), talin-2 (A19810), and BCAT2 (A7426) antibodies were purchased from ABclonal. The vinculin (ab129002), BCKDHA (ab305168), p-BCKDHA (S293) (ab302504) antibodies were purchased from Abcam. All antibodies were used following the manufacturer’s instructions.

The β-actin (1:1000, sc-130656) and BCKDK (E-12) (1:1000, sc-374425) antibodies were purchased from Santa Cruz Technology, Inc. (Santa Cruz, CA, USA). Flag antibody (1:1000, #F1804, #F7425) was purchased from Sigma-Aldrich (St. Louis, MO, USA). His Mouse monoclonal antibody (1:1000, 4E6) was obtained from Pregene, Inc (Beijing, China). All the following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): p-BCKDHA (S293) (1:500, #40368), BCKDHA (1:500, #90198), MEK1/2 (1:1000, #8727), p-MEK1/2 (S221) (1:1000, #2338), p-ERK1/2 (T202/Y204) (1:2000, #4370), ERK1/2 (1:2000, #4695), p-RPS6 (S235/236) (1:1000, #2211), p-RPS6 (S240/244) (1:1000, #2215), RPS6(1:1000, #2217), E-cadherin (1:500, #3195), N-cadherin (1:500, #13116), and Vimentin (1:500, #5741). p-BCKDK (Y246) antibody was prepared by Abiocode, Inc (shanghai, China). Rabbit (1:3000, #E030120) and mouse (1:3000, #E030110) antibodies were obtained from EarthOx Life Sciences (San Francisco, CA, USA). We purchased BT2 (Cat No. ZC-26488) from ZZBIO. CO.LTD (Shang Hai, China). Dasatinib (Cat No. S1021) was purchased from Selleckchem, Inc (Houston, TX, USA).