Dmem f12 medium base

Manufactured by Thermo Fisher Scientific

Sourced in United States

DMEM/F12 medium base is a cell culture medium formulation designed to support the growth and maintenance of a wide range of cell types. It provides a balanced combination of nutrients, salts, and other components required for cell viability and proliferation.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Eclipse ti microscope, by Nikon (1 mentions)

3 protocols using dmem f12 medium base

Time-Lapse Imaging of Ovarian Cell Migration

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Immortalized Ovarian Surface Epithelium cells (IOSE; from Dr. Susan Huang, MD Anderson Cancer Center) [60 (link)] were cultured at 37°C and 5% CO2 in DMEM/F12 medium base (LifeTechnologies 11330) supplemented with 10% FBS (LifeTechnologies 10082).
Prior to cell seeding, the fabricated scaffolds were sterilized with 1X PBS containing 100 U/mL Penicillin-Streptomycin (Invitrogen 15140–122). Cells were seeded at a density of 50K cell/mL and incubated overnight. Time-lapse imaging of migration was then performed by phase contrast imaging (10× 0.25NA objective; Nikon Ti-Eclipse microscope with Pathology Devices, Inc. - LiveCellTM incubator system). Phase-contrast images of each seeded scaffold were collected at 30 minutes intervals over 72 hours. Cells became too densely populated at longer times to isolate the cell-matrix interactions. At least 60–80 cells were used for each cell/scaffold combination.

Alkmin S., Brodziski R., Simon H., Hinton D., Goldsmith R.H., Patankar M, & Campagnola P.J. (2019). Migration dynamics of ovarian epithelial cells on micro-fabricated image-based models of normal and malignant stroma. Acta biomaterialia, 100, 92-104.

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Ovarian Cancer Cell Migration Dynamics

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Three ovarian epithelial cell lines of varying characteristics were used in this study: HEY (highly metastatic; from Dr. Molly Brewer, UCONN Health Center), OVCA433 (moderately metastatic; co-author Dr. Manish Patankar, WI, USA), and Immortalized Ovarian Surface Epithelium (IOSE; from Dr. Molly Brewer, MD, UCONN Health Center) as the normal control [53 (link),54 (link)]. These three cell lines were cultured at 37 °C with 5% CO₂ in DMEM/F12 medium base (LifeTechnologies 11330, Carlsbad, CA, USA) supplemented with 10% FBS (LifeTechnologies 10082).
Following fabrication and prior to cell seeding, the scaffolds were sterilized with 1X PBS containing 100 U/mL penicillin–streptomycin (Invitrogen 15140-122, Carlsbad, CA, USA). All cell lines were seeded at a density of 50K cell/mL and incubated overnight. Time-lapse studies were then performed by phase-contrast imaging (Nikon Ti-Eclipse inverted microscope with Pathology Devices, Inc., LiveCell^TM incubator system). Phase-contrast images (10×, 0.25NA objective) of each seeded scaffold were collected at 30 min intervals over 72 h. Cells became too densely populated at longer times to isolate the cell–matrix interactions, and collective migration events were not tracked. At least three independent measurements were used for each cell/scaffold combination with 60–80 attached cells in each case.

Alkmin S., Brodziski R., Simon H., Hinton D., Goldsmith R.H., Patankar M, & Campagnola P.J. (2020). Role of Collagen Fiber Morphology on Ovarian Cancer Cell Migration Using Image-Based Models of the Extracellular Matrix. Cancers, 12(6), 1390.

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Ovarian Cancer Cell Migration on Scaffolds

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Immortalized Ovarian Surface Epithelium (IOSE; from Dr. Molly Brewer, UCONN Health Center) and OVCA433 (moderately metastatic; co-author Dr. Manish Patankar, Wisconsin) were cultured at 37°C and 5% CO2 in DMEM/F12 medium base (LifeTechnologies 11330, Carlsbad, CA, USA) supplemented with 10% FBS (LifeTechnologies 10082).
Before cell seeding, the fabricated scaffolds were sterilized with 1X PBS containing 100 U/mL Penicillin-Streptomycin (Invitrogen 15140-122, Carlsbad, CA, USA). Cells were seeded at a density of 50K cell/mL and incubated overnight. Time-lapse imaging of migration was then performed by phase contrast imaging (10x 0.25NA objective; Nikon Ti-Eclipse microscope with Pathology Devices, Inc. - LiveCellTM incubator system). The incubator used for the time lapse measurements has 6 wells and each run included different combinations of cells and stiffnesses. Phase-contrast images of each seeded scaffold were collected at 30 minutes intervals over 72 hours. Cells became too densely populated at longer times to isolate the cell-matrix interactions and collective migration events were not tracked. At least 45-60 cells were used for each cell/scaffold combination.

Li J., Kasper D.L., Ausubel F.M., Rosner B, & Michel J.L. (1997). Inactivation of the α C protein antigen gene, bca, by a novel shuttle/suicide vector results in attenuation of virulence and immunity in group B Streptococcus. Proceedings of the National Academy of Sciences of the United States of America, 94(24), 13251-13256.

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