Mini protean tgx precasted gels

Manufactured by Bio-Rad

Sourced in United States

The Mini-PROTEAN TGX precast gels are laboratory equipment designed for performing polyacrylamide gel electrophoresis (PAGE). They provide a convenient and consistent platform for separating and analyzing proteins based on their molecular weight.

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Lab products found in correlation

Horseradish peroxidase coupled secondary antibody, by Cell Signaling Technology (1 mentions) Tgf βrii, by Abcam (1 mentions) Smad2 3, by Cell Signaling Technology (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) P jak2, by Cell Signaling Technology (1 mentions) P stat5, by Cell Signaling Technology (1 mentions) Stat5, by Santa Cruz Biotechnology (1 mentions) P smad2 3, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Trans blot turbo system, by Bio-Rad (1 mentions)

2 protocols using mini protean tgx precasted gels

Tau Protein Aggregation Regulation

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PERK^+/+ and PERK^−/−; eIF2α^S/S and eIF2α^A/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wildtype and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors [catalog no.: 11836153001; Roche]) or RIPA buffer. Protein concentrations of the cell lysates were determined by bicinchoninic acid protein assay (Pierce). Equal amounts of protein were loaded onto 4 to 15% Mini-PROTEAN TGX precasted gels (Bio-Rad) and immunoblotted. The following antibodies and dilutions were used: anti-HT-7 at 1:1000 dilution, AT8 at 1:1000 dilution, YFP at 1:1000 dilution, HSP90 at 1:2000 dilution, lamin A/C at 1:3000 dilution, GAPDH at 1:3000 dilution, T-PERK at 1:1000 dilution, phosphorylated PERK at 1:1000 dilution, eIF2α at 1:1000 dilution, and p-eIF2α at 1:1000 dilution. After overnight incubation with primary antibody, membranes were washed in Tris-buffered saline with 0.1% Tween-20, followed by incubation of a horseradish peroxidase–coupled secondary antibody (Cell Signaling). Immunoreactivity was detected using the SuperSignal West chemiluminescent substrate (Pierce) and BIO-RAD Universal Gel Molecular Imager.

Park G., Xu K., Chea L., Kim K., Safarta L., Song K.H., Wu J., Park S., Min H., Hiramatsu N., Han J, & Lin J.H. (2022). Neurodegeneration risk factor, EIF2AK3 (PERK), influences tau protein aggregation. The Journal of Biological Chemistry, 299(2), 102821.

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Evaluating TGF-β Signaling in Gata1low Mice

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BM and spleen from Gata1^low mice treated for 5 days were dissolved in lysis buffer containing protease and phosphatase inhibitors and stored at −80°C. Protein extracts were separated by electrophoresis under denaturing conditions using 7.5–10% mini-Protean TGX pre-casted gels (Bio-Rad, CA, USA) and transferred to nitrocellulose filters with the Transblot-Turbo system (Bio-Rad, Hercules). Filters were probed with antibodies against proteins of the canonical (SMAD2/3, cat no. 8685, Cell Signaling, Boston, MA, USA), p-SMAD2/3 (cat no. 8828, Cell Signaling), TGF-βRII (cat no. ab186838, Abcam, Cambridge, UK) and non-canonical (p38, cat no. 9212; p-p38, cat no. 4511; ERK1/2, cat no. 9102; p-ERK1/2, cat no. 9101; all from Cell Signaling) TGF-β signaling and of the JAK/STAT signaling (JAK2 (cat. No 3230, Cell Signaling), STAT5 (cat no. sc-74442, Santa Cruz, Dallas, Texas, USA), pJAK2 (Phospho-Tyr1007/1008 JAK2, cat no. 3771, Cell Signaling) and p-STAT5 (cat no, 9351, Cell Signaling). GAPDH (cat no. G9545, Sigma Aldrich) was used as a loading control. The bands were quantified with the ImageJ 1.52q software (National Institutes of Health, Bethesda, MD, USA) and normalized against GAPDH. Stoichiometry determinations of phospho-proteins levels were obtained by normalizing the content of the phosphoprotein with that of the corresponding total protein.

Hardy L.W, & Nalivaika E. (1992). Asn177 in Escherichia coli thymidylate synthase is a major determinant of pyrimidine specificity.. Proceedings of the National Academy of Sciences of the United States of America, 89(20), 9725-9729.

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