APN, Ang1, PLCγ and PKCα antibodies were purchased from Santa Cruz Biotechnology (CA, USA). p-PLCγ and p-PKCα antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). All siRNAs (ON-TARGETplus) were obtained from Dharmacon Research (Lafayette, CO, USA). Taqman® one-step PCR Master Mix, qPCR primers and probes were obtained from Applied Biosystems (Foster City, CA, USA). β-Actin antibody and pharmacological inhibitors were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Pharmacological inhibitors
Manufactured by Merck Group
Sourced in United States
Pharmacological inhibitors are a class of laboratory equipment used to modulate the activity of specific molecular targets, such as enzymes or signaling pathways, in a controlled and reproducible manner. These tools allow researchers to study the functional roles and mechanistic underpinnings of the targeted molecules within biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Taqman one step pcr master mix, by Thermo Fisher Scientific (2 mentions)
Qpcr primers, by Thermo Fisher Scientific (2 mentions)
β actin antibody, by Merck Group (2 mentions)
P plcγ, by Cell Signaling Technology (1 mentions)
P pkcα, by Cell Signaling Technology (1 mentions)
Ang 1, by Santa Cruz Biotechnology (1 mentions)
Bovine serum albumin (bsa), by Santa Cruz Biotechnology (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride, by Thermo Fisher Scientific (1 mentions)
Gf109203x, by Merck Group (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Fluospheres carboxylate modified microsphere, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Cytochalasin d, by Merck Group (1 mentions)
Vegf sc 7269, by Santa Cruz Biotechnology (1 mentions)
3 protocols using pharmacological inhibitors
1
Investigating Ang1-Mediated Signaling Pathways
2
Inhibition of Bacterial Infection Mechanisms
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Cells were pretreated for 1 h with the following pharmacological inhibitors (Sigma-Aldrich): kanamycin (30 μg/ml), ampicillin (100 μg/ml), chloramphenicol (5 μg/ml), CK-636 and CK-666 (50–150 μM), GF109203x (0.05–1.0 μM), and Gö6983 (0.5 μM). Cells were pretreated for 10 min with cytochalasin D (1 μM; Sigma-Aldrich). Antibodies include the following: anti–L. monocytogenes rabbit polyclonal antibodies (GeneTex); goat anti-rabbit secondary antibodies conjugated to Alexa488 and Alexa568 (Molecular Probes); anti-BSA rabbit antiserum (B1520; Sigma-Aldrich); mouse monoclonal antibodies directed against Rac1, RhoA, and Cdc42 (clones ARC03, ARH04, and ACD03, respectively; Cytoskeleton); rabbit anti-actin (clone A2103; Sigma-Aldrich); and secondary goat anti-mouse immunoglobulin G (IgG) and goat anti-rabbit IgG antibodies conjugated to horseradish peroxidase (Cell Signaling). FluoSpheres carboxylate modified microspheres (1 μm) (Molecular Probes), BSA (Santa Cruz Biotechnology), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Thermo-Fisher) were used to prepare the beads for LLO coating. Lipofectamine RNAiMax and Lipofectamine 2000 (Life Technologies) were used to transfect siRNA and plasmid DNA, respectively, using the manufacturer’s protocols. Silencer select siRNA for Rac1, RhoA, Cdc42, and NC were used at 60–90 pg per well in a six-well format (Life Technologies).
3
Signaling Pathway Antibody Optimization
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PLC (SC-58407), MEK (SC-6250), c-Jun (SC-74543), and VEGF (SC-7269) antibodies were purchased from Santa Cruz Biotechnology (CA, USA). p-PLC (2821S), p-MEK (2338S), and p-c-Jun (3270S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). All siRNAs (ON-TARGETplus) were obtained from Dharmacon Research (Lafayette, CO, USA). Taqman® one-step PCR Master Mix, qPCR primers, and probes were obtained from Applied Biosystems (Foster City, CA, USA). Beta-actin antibodies and pharmacological inhibitors were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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