Anti sma clone 1a4

The antibodies used for immunoblot analysis included IL-1β (1:1,000; R&D Systems), GROα (1:1,000; Thermo Fisher Scientific), IL-6 (1:1,000; R&D Systems), IL-8 (1:1,000; R&D Systems), IL-1R1 (1:1,000; R&D Systems), phosphorylated ERK (pERK; 1:5,000; Sigma-Aldrich), β-tubulin (1:5,000; Santa Cruz Biotechnology, Inc.), ERK2 (1:5,000; Santa Cruz Biotechnology, Inc.), pp65 (1:1,000; Cell Signaling Technology), p65 (1:1,000; Cell Signaling Technology), and BCL2 (1:1,000; Cell Signaling Technology). Anti–rabbit IgG-HRP (1:5,000) and anti–mouse IgG-HRP (1:5,000) were obtained from GE Healthcare, and anti–goat IgG-HRP (1:2,000) was obtained from Santa Cruz Biotechnology, Inc. The primary antibodies used for immunohistochemical analysis included anti–IL-1β (goat polyclonal; 1:50; R&D Systems), anti–IL-1R1 (goat polyclonal; 1:50; R&D Systems), anti-CD68 (clone KP1; mouse; 1:300; Dako), anti-CD163 (clone 10D6; mouse; 1:50; Thermo Fisher Scientific), anti-SOX10 (goat polyclonal; 1:120; Santa Cruz Biotechnology, Inc.), anti–IBA-1(rabbit polyclonal; 1:300; Wako Pure Chemical Industries), anti-SMA (clone 1A4; mouse; 1:200; Thermo Fisher Scientific), and anti-GROα (rabbit polyclonal; 1:50; Proteintech) followed by appropriate detection systems.