Thermosensitive alkaline phosphatase
Thermosensitive alkaline phosphatase is an enzyme designed for use in molecular biology applications. It catalyzes the removal of phosphate groups from various molecules, including nucleic acids and proteins. The enzyme is thermosensitive, meaning its activity is temperature-dependent.
Lab products found in correlation
6 protocols using thermosensitive alkaline phosphatase
Generation of Csf1r-mApple reporter mice
Identification of LATS1 Phosphorylation Motifs
Preparation of Precursor tRNAs from Physcomitrella
For preparation of chloroplast pre-tRNAArg isoacceptors, the corresponding genes were cloned and in vitro transcribed. The obtained pre-tRNAs were dephosphorylated with Thermo Sensitive Alkaline Phosphatase (Promega) and were 5′-end radiolabeled with [γ32P] ATP and polynucleotidekinase (TaKaRa). Labeled RNAs were purified from the gel after 8% polyacrylamide gel electrophoresis (PAGE) containing 7M urea.
Plasmid Preparation and Enzymatic Manipulation
Quantifying Kinase Activity Using Isotope-Labeled Formaldehyde
Codon-Optimized Heterologous Gene Expression in L. lactis
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