Multi myograph model 810ms

Fresh prostate specimens obtained from patients (or brain-dead men) were cut into vertical strips of the same length, approximately 1 × 0.5 × 0.5 cm, and installed lengthwise in a 10 mL organ bath (Multi-Myograph Model 810MS; Danish Myo Technology, DEN). The myograph was connected in line to a PowerLab 4/30 Data Acquisition System (ADInstruments, USA) and in turn to a Dual-Core processor Pentium computer for real-time monitoring of physiological force. The prostate strips were equilibrated at least 1 h in Krebs buffer at 37 °C with continuous bubbling of 95% O₂ and 5% CO₂. The buffer was composed of (in mM): NaCl 110, KCl 4.8, CaCl₂ 2.5, MgSO₄ 1.2, KH₂PO₄ 1.2, NaHCO₃ 25 and dextrose 11. During the organ bath experiments, the Krebs buffer was changed every 10 min. During the process of equilibration, the tension of the strips was continuously adjusted to 2000 mg. After equilibration, prostate strips were contracted with phenylephrine (PE, Grandpharma, CHN) (10⁻⁵M). They then were completely washed out and pre-incubated with 10 μM Y-27632 (MedChemExpress, CHN) for 15 min. Then, they were contracted with the same dose of PE. The inhibitory effect of Y-27632 was calculated.

As previously described [12 (link),16 (link),18 (link)], rat and human prostate strips were mounted longitudinally in a 4 mL organ bath, Multi Myograph Model 810MS (Danish Myo Technology; Aarhus, Denmark). The myograph was connected in line to a PowerLab 4/30 Data Acquisition System (ADInstruments; Colorado Springs, CO, USA) and then to a Pentium Dual-Core processor computer, which allowed us to monitor the physiological force in real time. The SM strips were equilibrated for at least 1 h in Krebs buffer at 37 °C with continuous bubbling of 95% O₂ and 5% CO₂. The buffer had the following mM composition: NaCl, 110; KCl, 4.8; CaCl₂, 2.5; MgSO₄, 1.2; KH₂PO₄, 1.2; NaHCO₃, 25; and dextrose, 11, with replacement every 15 min. The strips were continuously adjusted to resting tension. After equilibration, the tissues were contracted using 60 mM KCl. This degree of contractile response was considered 100%, and the force induced by different concentrations of phenylephrine (PE) was expressed as a percentage of this value.