16 hydroxyhexadecanoic acid

Manufactured by Merck Group

Sourced in United States

16-hydroxyhexadecanoic acid is a long-chain fatty alcohol that can be used in various laboratory applications. It is a white crystalline solid at room temperature. The core function of this compound is to serve as a chemical building block for further synthesis and analysis in a laboratory setting.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Diisopropyl ether, by Merck Group (1 mentions) Pentasodium triphosphate, by Merck Group (1 mentions) Celllight late endosomes gfp, by Thermo Fisher Scientific (1 mentions) Silver enhancer kit, by Merck Group (1 mentions) Er tracker green, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 nhs ester, by Thermo Fisher Scientific (1 mentions) Celllight early endosomes gfp, by Thermo Fisher Scientific (1 mentions) Lysotracker green dnd 26, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Chitosan, by Merck Group (1 mentions)

3 protocols using 16 hydroxyhexadecanoic acid

Inducing Filament and Appressoria in Moesziomyces

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Haploid strains of MbA were grown in liquid cultures, mixed, and drops arranged on PD plates with charcoal to induce filament formation. Plate with the haploid U. maydis strains FB1 and FB2 and the solopathogenic strain SG200 served as internal control.
The complete b-locus of the solopathogenic U. hordei strain DS200 was amplified (Figure 1—figure supplement 2) and inserted into the MbA b-locus by homologous recombination. The strain obtained, known as compatible b1 (CB1), was tested positive by amplification of the right border and left border areas with primers specific for the genomic locus and for the plasmid region. Additionally, two primers specific for the MbA bE and bW genes were chosen to amplify parts of the native locus. To induce filament and appressoria formation in vitro we used a Moesziomyces YEPSL culture at OD₆₀₀0.6–0.8. The cells were diluted to an OD₆₀₀ of 0.2 in 2% YEPSL (for appressoria formation 100 µM 16-hydroxyhexadecanoic acid [Sigma-Aldrich] or 1% ethanol was added) and sprayed the yeast like cells on parafilm which mimics the hydrophobic plant surface. After 18 hr of incubation at 100% humidity the number of cells grown as filaments (or generating appressoria) was determined relative to the total number of total cells by using a light microscope.

Eitzen K., Sengupta P., Kroll S., Kemen E, & Doehlemann G. (2021). A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme. eLife, 10, e65306.

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Ex4-Loaded Chitosan Nanoparticles for Insulin Delivery

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Ex4 was purchased from GL Biochem Ltd. (Shanghai, China). RPMI 1640, fetal bovine serum, rabbit anti-murine and human FATP4 polyclonal antibody (PA5–42446), Alexa Fluor 594–NHS Ester, LysoTracker Green DND-26, CellLight Early Endosomes–GFP, CellLight Late Endosomes–GFP, and ER-Tracker Green were obtained from Thermo Fisher (Waltham, MA, USA). Rabbit anti-murine insulin antibody was from Abcam (ab63820, Cambridge, MA, USA). Chitosan (molecular weight, 50,000 to 190,000 Da), pentasodium triphosphate, zinc trifluoroacetate hydrate, 16-hydroxyhexadecanoic acid, silver enhancer kit, and diisopropyl ether were ordered from Sigma-Aldrich (St. Louis, MO, USA). Mono-Sulfo-NHS-Nanogold (1.4 nm) was purchased from Nanoprobe, Inc. (Yaphank, NY, USA), and 1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt) and cholesterol were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA).

Hu Z., Nizzero S., Goel S., Hinkle L.E., Wu X., Li C., Ferrari M, & Shen H. (2020). Molecular targeting of FATP4 transporter for oral delivery of therapeutic peptide. Science Advances, 6(14), eaba0145.

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Filament and Appressoria Formation in Moesziomyces

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Haploid strains of MbA were grown in liquid cultures, mixed and drops arranged on PDplates with charcoal to induce filament formation. Plate with the haploid U. maydis strains FB1 and FB2 and the solopathogenic strain SG200 served as internal control.
The complete b-locus of the solopathogenic U. hordei strain DS200 was amplified (Figure S2) and inserted into the MbA b-locus by homologous recombination. The strain obtained, known as compatible b1 (CB1) was tested positive by amplification of the right border and left border areas with primers specific for the genomic locus and for the plasmid region. Additionally, two primers specific for the MbA bEand bW genes were chosen to amplify parts of the native locus. To induce filament and appressoria formation in vitro we used a Moesziomyces YEPSL culture at OD 600 0.6-0.8. The cells were diluted to an OD 600 of 0.2 in 2% YEPSL (for appressoria formation 100µM 16hydroxyhexadecanoic acid (Sigma-Aldrich) or 1% ethanol was added) and sprayed the yeast like cells on parafilm which mimics the hydrophobic plant surface. After 18h incubation at 100% humidity the number of cells grown as filaments (or generating appressoria) was determined relative to the total number of total cells by using a light microscope.

Eitzen K., Sengupta P., Kroll S., Kemen E., & Doehlemann G. (2020). A fungal member of theArabidopsis thalianaphyllosphere antagonizesAlbugo laibachiivia a secreted lysozyme.

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