Rpmi 1640 w glutamax

The colorectal cancer cell line HCT116 was obtained from Jens Schmid (Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology, Stuttgart, Germany). HT29/GFP-luciferase cells were obtained from Julia Beil (University Hospital Tübingen, Eberhard Karls University, Internal Medicine VIII, Tübingen, Germany). Cell lines were authenticated by STR analysis (Eurofins, Ebersberg, Germany). For quality control, mycoplasma PCR using the Venor^®GeM Classic kit (11-1025, Minerva Biolabs, Berlin, Germany) was performed according to the manual and before the use of cell lines. HCT116 cells were cultured in RPMI 1640 w/Glutamax (Thermo Fisher Scientific GmbH, Waltham, Massachusetts, US) supplemented with 1% penicillin/streptomycin (Biochrom AG, Berlin, Germany) and 10% FCS (Thermo Fisher Scientific GmbH, Waltham, Massachusetts, US) in a 5% CO₂ incubator at 37 °C. HT29/GFP-luciferase cells were cultured in McCoy’s 5A Medium w/l-glutamine (Pan Biotech GmbH, Aidenbach, Germany) supplemented with 1% penicillin/streptomycin and 10% FCS in a 5% CO₂ incubator at 37 °C. For coculture experiments 2,500–5,000 cells were seeded onto the tissue. After 24 h medium replaced with fresh medium to remove cells that did not attach to the tissue.

The Colo205/GFP CRC cell line was purchased from Cellomics Technology (SC-1278; Halethorpe, MD, USA) and the HT29/GFP-luciferase and SKOV3/GFP cell lines were obtained from Julia Beil (University Hospital Tübingen, Eberhard Karls University, Internal Medicine VIII, Tübingen, Germany). Cell lines were authenticated by short tandem repeat analysis (Eurofins, Ebersberg, Germany). The cell lines were tested for mycoplasma by polymerase chain reaction (PCR) using a Venor^®GeM Classic kit (11-1025; Minerva Biolabs, Berlin, Germany) according to the manufacturer’s instructions before use in experiments. Colo205/GFP cells were cultivated in RPMI 1640 w/Glutamax (Thermo Fisher Scientific GmbH, Waltham, MA, USA) supplemented with 1% penicillin/streptomycin (Biochrom AG, Berlin, Germany) and 10% fetal calf serum (FCS) (Thermo Fisher Scientific GmbH, Waltham, MA, USA) in a 5% CO₂ incubator at 37 °C. HT29/GFP-luciferase and SKOV3/GFP cells were cultivated in McCoy’s 5A Medium with L-glutamine (Pan Biotech GmbH, Aidenbach, Germany) supplemented with 1% penicillin/streptomycin and 10% FSC in a 5% CO₂ incubator at 37 °C.