The ABTS free radical scavenging activity of CMJE was measured by using the ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) radical cation decolorization assay [17 (link)]. ABTS•+ was generated by reacting 7 mM ABTS aqueous solution with 2.45 mM potassium persulfate in the dark for 12-16 h at room temperature. At the beginning of the assay, this solution was diluted in ethanol (about 1 : 49, v/v) and equilibrated at 30 ± 2°C to give an absorbance of 0.7 ± 0.02 at 734 nm. The stock solution of the CMJE extract was diluted to yield a concentration range of 50-8000 μg/mL. The final concentration (0-15 μM) was obtained by the addition of 1 mL of the diluted ABTS•+ solution to 62 μL of CMJE sample in ethanol. After 40 minutes of mixing, absorbance was estimated at 25°C. Trolox and ethanol were used as a positive control and blank, respectively. At each dilution of the standard and sample, triplicate determinations were made, and absorption was measured at 734 nm in the UV-Vis spectrophotometer (UV-1200S UV-VIS 1200, Shimadzu Corporation, Japan). The ABTS•+ scavenging capability of the extract was compared with that of Trolox. The percentage inhibition calculated as follows:
Uv 1200s uv vis 1200
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The UV-1200S UV-VIS 1200 is a spectrophotometer designed for ultraviolet and visible light absorption measurements. It is capable of performing various spectroscopic analyses, including quantitative determination of chemical concentrations and identification of organic and inorganic compounds.
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ABTS Radical Scavenging Activity of CMJE
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Assessing Antioxidant Potential via ABTS Assay
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The ABTS (2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical cation decolorization test [13] was used to assess LSM/LSE's free radical scavenging capability. 7 mM ABTS aqueous solution was mixed with 2.45 mM potassium persulfate in the dark for 12-16 hours at room temperature to produce ABTS•+. This solution was diluted in ethanol (approximately 1: 49, v/v) and equilibrated at 30 2°C to yield an absorbance of 0.7 0.02 at 734 nm at the start of the test. Next, the LSM/LSE extract stock solution was diluted to give a concentration range of 50-8000 g/mL. By adding 1 mL of diluted ABTS•+ solution to 62 L of LSM/LSE sample in ethanol, the nal concentration (0-15 M) was reached. The absorbance was read at 25°C after 40 min of mixing. Positive control and a blank were employed with Trolox and ethanol. Triplicate determinations were made at each dilution of the standard and sample, and absorption was measured in the UV-Vis spectrophotometer at 734 nm (UV-1200S UV-VIS 1200, Shimadzu Corporation, Japan). The extract's ABTS•+ scavenging capacity was matched to that of Trolox. Percentage inhibition is computed as follows:
ABTS radical scavenging activity = [(Absorbance of control -Absorbance of the sample) / Absorbance of control] x100
ABTS radical scavenging activity = [(Absorbance of control -Absorbance of the sample) / Absorbance of control] x100
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