The largest database of trusted experimental protocols

Anti pgk1 22c5d8

Manufactured by Abcam
Sourced in United States

Anti-Pgk1 (22C5D8) is a mouse monoclonal antibody that recognizes the Phosphoglycerate kinase 1 (Pgk1) protein. Pgk1 is a key enzyme in the glycolytic pathway. The antibody can be used to detect and study the expression of Pgk1 in various biological samples.

Automatically generated - may contain errors

3 protocols using anti pgk1 22c5d8

1

Quantitative Immunoblot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction with trichloroacetic acid, immunoblotting, and protein detection by immunoblotting using a Li-Cor Odyssey infrared dye detection system were performed as previously described (20 (link)). Protein abundance was quantified from immunoblots using Image Studio version 5.2.5 (LiCor) (licor.com/bio/image-studio/). Rabbit anti-Pyk1 antibody (69 (link)) was a gift of Jeremy Thorner, and rabbit anti-Fba1 antibody (70 (link)) a gift of Magdalena Boguta. Anti-Pgk1 (22C5D8) was obtained from Abcam. IRDyeR 680LT dye-labeled anti-mouse antibody and IRDyeR 800CW dye-labeled anti-rabbit antibody were obtained from Li-Cor. Antibody specificity in immunoblots was verified by comparison of GFP-tagged and untagged target molecular weight via immunoblotting, and/or by comparison of protein samples from WT and deletion mutant strains.
+ Open protocol
+ Expand
2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
TCA protein extracts were prepared, and an equal amount of each sample was separated by SDS-PAGE (44 (link)). Western blottings were performed with anti-Rad53 (kind gift by C. Santocanale), anti-HIS-tag (70796-3 Novagen, Merck, Darmstadt, Germany) or anti-miniAID-tag (MBL International, Woburn, MA, USA (43 )) or anti-Pgk1 (22c5d8 Abcam, Cambridge, UK) antibodies, using standard techniques.
+ Open protocol
+ Expand
3

Western Blotting Analysis of Myc-tagged Psa1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were obtained as described in Kushnirov (2000) (link), resolved on SDS 10% polyacrylamide electrophoresis gels, and analyzed by Western blotting according to standard procedures. Myc-tagged Psa1 was probed with anti-c-Myc (9E10; Santa Cruz Biotechnology) at 1:1000 dilution and Pgk1 with anti-PGK1 (22C5D8; Abcam) at 1:5000 dilution. In both cases, mouse IgG kappa-binding protein HRP (Santa Cruz Biotechnology) at 1:50,000 dilution was used to detect the primary antibody. Blots were developed using Biorad Clarity Western ECL substrates and visualized with a C-DiGit Blot scanner (LI-COR Biosciences). Band intensity was quantified with GelAnalyzer 19.1 software and the results were normalized to Pgk1. The level of protein was expressed relative to its abundance in wild-type cells at 30°C, which was set as 1. Three independent protein extractions were analyzed for each sample.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!