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Lactate assay kit

Manufactured by Thermo Fisher Scientific
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The Lactate Assay Kit is a colorimetric assay designed to quantify lactate levels in various biological samples. The kit provides a simple and accurate method to measure lactate concentration without the need for extensive sample preparation.

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Lab products found in correlation

Amplex red glucose glucose oxidase assay kit, by Thermo Fisher Scientific (1 mentions) Atp assay kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using lactate assay kit

1

Glucose and Lactate Quantification in Cancer Cells

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Either the target plasmid or miRNAs mimics were transfected into MDA-MB-231 or MDA-MB-468 cells. Cell culture media were collected after transfection for 48 h. Glucose uptake and lactate production were measured using Amplex® Red Glucose/Glucose Oxidase Assay Kit (Cat. A22189, Invitrogen) and lactate assay kit (Cat. MAK064, Sigma Aldrich) respectively. The results were normalized on the basis of total cellular protein amounts.
Liu P., Ye F., Xie X., Li X., Tang H., Li S., Huang X., Song C., Wei W, & Xie X. (2016). mir-101-3p is a key regulator of tumor metabolism in triple negative breast cancer targeting AMPK. Oncotarget, 7(23), 35188-35198.
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2

Metabolic Profiling of CRC Cells

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Either the pENTR-miR-26a or miR-26a inhibitor was transfected into CRC cells. Cell culture media were collected after transfection for 48 h. Glucose uptake and lactate production were measured using Amplex® Red Glucose/Glucose Oxidase Assay Kit (Cat. #A22189; Invitrogen) and lactate assay kit (Cat. #MAK064; Sigma-Aldrich) respectively. The results were normalized on the basis of total cellular protein amounts.
Chen B., Liu Y., Jin X., Lu W., Liu J., Xia Z., Yuan Q., Zhao X., Xu N, & Liang S. (2014). MicroRNA-26a regulates glucose metabolism by direct targeting PDHX in colorectal cancer cells. BMC Cancer, 14, 443.
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3

Metabolic Profiling of GC Cells

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The Glucose Assay kit, Lactate Assay Kit and ATP Assay kit (Invitrogen, USA) were used to determined the GC cells glucose consumption, Adenosine Triphosphate (ATP) Synthesis and Lactate Production. All measurements followed manufacturer's instructions and were normalized for the number of cells in each experiment.
Jin L., He Z., Zhu C., Xiao G., Yang X., Chen X., & Huang W. (2020). The circBVES Acts as a Sponge of miR-145-5p to Promote Gastric Cancer Glycolysis and Progression Through Regulating HMGB3 Expression.
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