Mice were deeply anesthetized with 5% isoflurane and transcardially perfused with ice-cold aCSF containing (in mM): KCl, 2.5; NaH2PO4, 1.25; HEPES, 20; N-Methyl-D-glucamine (NMDG), 93; L-ascorbic acid (sodium salt), 5; thiourea, 2; sodium pyruvate, 3; CaCl2, 0.5; MgSO4, 10; D-glucose, 25; N-acetyl-L-cysteine, 12; NaHCO3, 30; with pH adjusted to 7.30 with HCl and osmolarity adjusted to 310 mOsm, and equilibrated with 95% O2 and 5% CO2. Slices recovered in modified aCSF for 30 min at 32°C. Slices were transferred to standard (recording) aCSF containing (in mM): NaCl, 125; KCl, 2.5; MgSO4, 2; NaH2PO4, 1.25; CaCl2, 2; D-glucose, 20; NaHCO3, 26. Slices were maintained at room temperature for up to 3 h before recording. Slices were then transferred to a recording chamber on the stage of an upright microscope (Olympus) and continuously perfused using a diaphragm pump (Smoothflow Pump Q Series, Model #Q-100-TT-ULP-ES, TACMINA CORPORATION, Japan) and suction (PC-21, NARISHIGE, Japan) with recording aCSF at a rate of 3 mL/min at 30 ± 1°C.
Pc 21
Manufactured by Narishige
Sourced in Japan
The PC-21 is a programmable microcontroller from Narishige. It is designed for precise control and monitoring of laboratory equipment. The device features user-programmable control parameters and data logging capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pc 21
1
Preparation of Acute Brain Slices
2
Preparation of Acute Brain Slices
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Mice were deeply anesthetized with 5% isoflurane and transcardially perfused with ice-cold aCSF containing (in mM): KCl, 2.5; NaH2PO4, 1.25; HEPES, 20; N-Methyl-D-glucamine (NMDG), 93; L-ascorbic acid (sodium salt), 5; thiourea, 2; sodium pyruvate, 3; CaCl2, 0.5; MgSO4, 10; D-glucose, 25; N-acetyl-L-cysteine, 12; NaHCO3, 30; with pH adjusted to 7.30 with HCl and osmolarity adjusted to 310 mOsm, and equilibrated with 95% O2 and 5% CO2. Slices recovered in modified aCSF for 30 min at 32°C. Slices were transferred to standard (recording) aCSF containing (in mM): NaCl, 125; KCl, 2.5; MgSO4, 2; NaH2PO4, 1.25; CaCl2, 2; D-glucose, 20; NaHCO3, 26. Slices were maintained at room temperature for up to 3 h before recording. Slices were then transferred to a recording chamber on the stage of an upright microscope (Olympus) and continuously perfused using a diaphragm pump (Smoothflow Pump Q Series, Model #Q-100-TT-ULP-ES, TACMINA CORPORATION, Japan) and suction (PC-21, NARISHIGE, Japan) with recording aCSF at a rate of 3 mL/min at 30 ± 1°C.
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