3 methyladenine 3ma sc 205596

Antibodies for GAPDH (#2118), phospho-Bcl-2 (#2827), phosphor-mTOR (#2976), Beclin1 (#3495), phospho-JNK (#4668), JNK (#9252) and Alexa Fluor 488-conjugated IgG (#4408) were purchased from Cell Signaling Technology (Cell Signaling Technology, Danvers, MA, USA). Anti-PARP and p62 (610497) were purchased from BD Biosciences (New York, USA). Antibodies for LC3B (L7543) and chloroquine (CQ, 50-63-5) were purchased from Sigma-Aldrich (St Louis, MO, USA). All HRP-conjugated IgG secondary antibodies were purchased from Abgent (San Diego, CA, USA). The JNK inhibitor SP600125 (s1460) and docetaxel (s1148) were purchased from Selleck (Houston, TX, USA). The 3-methyladenine (3MA) (sc-205596) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TP (84650-60-2) was purchased from Yongye Biotechnology (Qingpu, Shanghai, China). Roswell Park Memorial Institute-1640 (RPMI-1640) and trypsin were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Gibco (Thermo Fisher Scientific, MA, USA).

Unless otherwise stated, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tissue Culture Medium-199 (TCM-199 containing l-glutamine and phenol red) and fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY, USA). Folltropin–V (Follicle-stimulating hormone; FSH) was purchased from Bioniche Animal Health Canada Inc. (Bellevile, Ontario, Canada) and Chorulon (Human chorionic gonadotrophin; hCG) from Intervet Schering Plough (Roseland, NJ, USA). EmbryoMax (KSOM Powdered Media Kit #MR-020P-5F) was purchased from MilliPore (Livingston, Fleming Road, UK). The autophagy inhibitor 3-Methyladenine (3MA; #sc-205596) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The primary antibody [LC3A/B (D3U4C) rabbit monoclonal antibody—#12741] and secondary antibody (anti-rabbit IgG – HRP-linked antibody—#7074) were purchased from Cell Signaling Technology (Danvers, 3 Trash Lane, MA). Frozen semen was purchased from CRV Lagoa (Sertãozinho, São Paulo, Brazil).