Mir 30b

Manufactured by Qiagen

Sourced in United States

The MiR-30b is a laboratory instrument designed to perform quantitative analysis of microRNA (miRNA) expression levels. It uses real-time PCR technology to accurately measure the abundance of specific miRNA molecules in biological samples. The core function of the MiR-30b is to provide reliable and reproducible miRNA quantification data.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Mir 24, by Qiagen (2 mentions) Mir 142 3p, by Qiagen (2 mentions) Mirna mimic, by Qiagen (1 mentions) Mir 27a, by Qiagen (1 mentions) Mir 26a, by Qiagen (1 mentions) Red siglo oligos, by Thermo Fisher Scientific (1 mentions) Control mirna mimic, by Qiagen (1 mentions)

Close spelling variants of this product

Primers for miR-30b and U5/U6

3 protocols using mir 30b

miRNA Transfection in Immune Cells

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MiR-24, miR-30b, and miR-142-3p mimics or inhibitors were purchased from Qiagen (Gaithersburg, MD, USA). As a negative control, miRNA mimics with no known human targets was used (Qiagen). Transient transfection of miRNA mimics, inhibitors or control was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). MΦ were transfected at a final concentration of 50 nM oligos while DC were transfected with 100 nM oligos. As a positive transfection control, we used DY-547 labelled siRNA (Life Technologies).

Naqvi A.R., Fordham J.B., Ganesh B, & Nares S. (2016). miR-24, miR-30b and miR-142-3p interfere with antigen processing and presentation by primary macrophages and dendritic cells. Scientific Reports, 6, 32925.

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Transfection of miRNA mimics in HEK293 and oral keratinocytes

Cited 1 time

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MiR-24, miR-26a, miR-27a, miR-30b or control mimics were purchased (Qiagen) and transfection was performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) in HEK293 and human oral keratinocytes as previously described (Naqvi et al., 2018 (link)).

Naqvi A.R., Brambila M.F., Martínez G., Chapa G, & Nares S. (2019). Dysregulation of human miRNAs and increased prevalence of HHV miRNAs in obese periodontitis subjects. Journal of clinical periodontology, 46(1), 51-61.

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Overexpressing miRNA in Differentiated Macrophages and Dendritic Cells

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miR-30b, miR-142–3p and control miRNA mimic were purchased from Qiagen (Gaithersburg, MD, USA). Transient miRNA overexpression was performed by transfecting miRNA mimics using Lipofectamine 2000 (Invitrogen-Life Technologies Corporation, Carlsbad, CA, USA) according to the manufacturer’s instructions. Day 7-differentiated MΦ or DC were transfected at a final concentration of 50 nM. Red siGLO oligos (ThermoFisher Scientific, Grand Island, NY, USA) were used to determine transfection efficiency.

Valverde A., Nares S, & Naqvi A.R. (2020). Impaired cell migration and structural defects in myeloid cells overexpressing miR-30b and miR-142–3p. Biochimica et biophysica acta. Gene regulatory mechanisms, 1863(11), 194628.

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