Rabbit anti phospho ser thr pka substrate

Manufactured by Cell Signaling Technology

Rabbit anti-Phospho-(Ser/Thr) PKA Substrate is a primary antibody that specifically recognizes proteins phosphorylated by Protein Kinase A (PKA) on serine or threonine residues. It is designed for use in Western blotting and other immunoassay applications to detect PKA substrate phosphorylation.

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Lab products found in correlation

Rabbit anti phospho creb ser133, by Cell Signaling Technology (1 mentions) Mouse anti gapdh, by OriGene (1 mentions) Mouse anti pparγ, by Santa Cruz Biotechnology (1 mentions) Mouse anti pgc1α, by Santa Cruz Biotechnology (1 mentions) Rabbit anti atgl, by Cell Signaling Technology (1 mentions) Guinea pig anti plin2, by Biosynth (1 mentions) Anti phospho hsl, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-(Ser/Thr) PKA Substrate (11 citations) Anti-PKA (9 citations) Anti-phospho-PKA substrate (8 citations) Phospho-PKA substrate (8 citations) Anti-phospho-(Ser/Thr) PKA substrate (7 citations) Anti-phospho Ser/Thr (5 citations) Anti-phospho-PKA (5 citations) Rabbit anti-phospho-PKA substrate (4 citations) Rabbit anti-PKA (3 citations)

2 protocols using rabbit anti phospho ser thr pka substrate

Western Blot Analysis of Protein Expression

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Western blot analysis was performed as previously reported (34 (link), 35 (link)). The primary antibodies used in Western blot analysis are mouse anti–PGC-1α (Santa Cruz, sc-517380), mouse anti-PPARγ (Santa Cruz, sc-7273), mouse anti-PTH1R (Santa Cruz, sc-12722), mouse anti–GAPDH (Origene, TA802519), rabbit anti- Phospho-(Ser/Thr) PKA Substrate (Cell Signaling Technology, 9621), rabbit anti- Phospho-CREB (Ser133) (Cell Signaling Technology, 9198), rabbit anti-CREB (Proteintech, 12208-1-AP).

Feng X., Xiao Y., Guo Q., Peng H., Zhou H.Y., Wang J.P, & Xia Z.Y. (2022). Parathyroid hormone alleviates non-alcoholic liver steatosis via activating the hepatic cAMP/PKA/CREB pathway. Frontiers in Endocrinology, 13, 899731.

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Antibody Procurement for Lipolytic Proteins

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Antibodies to lipolytic effector proteins can be purchased from (or produced by) a variety of commercial sources: anti-phospho-HSL (Cell Signaling Technology, Cat. # 4139), guinea pig anti-PLIN2 (Fitzgerald Industries International), goat anti-PLIN1 (Everest Biotech, EB07728), rabbit anti-ATGL (Cell Signaling Technology, Cat. # 2439), and rabbit anti-phospho-(Ser/Thr) PKA substrate (Cell Signaling Technology, Cat. # 9621). Our lab has found it cost-effective to contract the production of affinity-purified polyclonal antibodies from commercial sources (Proteintech, Chicago, IL). HCS LipidTOX Deep Red (Cat. # H34477), Bodipy FL C₁₆ (Cat. # D3821), and MitoTracker Red CMXRos (Cat. # M-7512) are purchased from Invitrogen.

Mottillo E.P., Paul G.M., Moore H.P, & Granneman J.G. (2014). Use of Fluorescence Microscopy to Probe Intracellular Lipolysis. Methods in enzymology, 538, 263-278.

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