N ras f155

Manufactured by Santa Cruz Biotechnology

N-Ras (F155) is a recombinant protein product designed for research purposes. It is a member of the Ras protein family, which are small GTPases involved in cell signaling pathways. The N-Ras (F155) product specifically refers to the F155 isoform of the N-Ras protein.

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Lab products found in correlation

Hras c 20, by Santa Cruz Biotechnology (1 mentions) Perk1 2t202 t204, by Cell Signaling Technology (1 mentions) Cabozantinib, by Selleck Chemicals (1 mentions) Afatinib, by Selleck Chemicals (1 mentions) Foretinib, by Selleck Chemicals (1 mentions) Ponatinib, by Selleck Chemicals (1 mentions) Pegfr y1068, by Cell Signaling Technology (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Trametinib, by Selleck Chemicals (1 mentions) Lc2 ad cells, by Merck Group (1 mentions) Perk1 2 xp t202 y204, by Cell Signaling Technology (1 mentions) Pegfr y1173, by Cell Signaling Technology (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Gapdh mab374 antibody, by Merck Group (1 mentions) Anti mouse igg hrp linked 7076 s, by Cell Signaling Technology (1 mentions) Anti rabbit igg hrp linked 7074s, by Cell Signaling Technology (1 mentions)

3 protocols using n ras f155

Ras and Ral Activation Assays

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Antibodies against β-actin and K-Ras were obtained from Sigma, H-Ras (c-20) and N-Ras (F155) from Santa Cruz Biotechnology, Ras and Ral-A from Upstate, M-Ras from Thermo Scientifics, R-Ras, AKT, p-AKT (S473), ERK1/2, p-ERK1/2 (T202/T204) from Cell Signaling Technology, and RASA1 from Epitomics. Active Ras-GTP and Ral-GTP levels were determined using Ras and Ral Activation Assay Kits (Millipore) respectively, following the manufacturer's instructions.

Sung H., Kanchi K.L., Wang X., Hill K.S., Messina J.L., Lee J.H., Kim Y., Dees N.D., Ding L., Teer J.K., Yang S., Sarnaik A.A., Sondak V.K., Mulé J.J., Wilson R.K., Weber J.S, & Kim M. (2016). Inactivation of RASA1 promotes melanoma tumorigenesis via R-Ras activation. Oncotarget, 7(17), 23885-23896.

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Cell Line Authentication and Kinase Inhibitor Assay

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LC-2/ad cells were obtained from Sigma (cat no. 94072247), TPC1 cells obtained from R.E. Schweppe (17 (link)); H2228 cells obtained from J.D. Minna. HCC78-TAER were previously described (18 (link)). Cells were maintained in RPMI-1640 (Invitrogen) with 10% FBS at 37°C in a humidified 5% CO₂ incubator. Fingerprint analysis of cell lines was performed bi-annually by the Molecular Biology Service Center at the Barbara Davis Center for Diabetes at the University of Colorado Anschutz Medical Campus in Aurora, CO to ensure authenticity. Alectinib was provided by Chugai Pharmaceuticals. Ponatinib, cabozantinib, trametinib, gefitinib, afatinib, and foretinib were obtained from Selleck Chemicals. Pervanadate was generated by incubating hydrogen peroxide with 100 mM sodium orthovanadate in distilled water. Antibodies used were as follows: pEGFR Y1068 (D7A5), pEGFR Y1173 (53A5), total RET (D3D8R), pERK1/2 XP T202/Y204 (D13.14.4E), total ERK1/2 (L34F12), pAKT S473 XP (D9E), total AKT (40D4), and pSHC1 Y239/Y240 (2434) from Cell Signaling; pTYR (4G10 Platinum), GAPDH (6C5) and GAPDH (ABS16) from Millipore; pRET Y1062, α-tubulin (TU-02) and NRAS (F155) from Santa Cruz Biotechnology.

Nelson-Taylor S.K., Le A.T., Yoo M., Schubert L., Mishall K.M., Doak A., Varella-Garcia M., Tan A.C, & Doebele R.C. (2017). Resistance to RET-inhibition in RET-rearranged NSCLC is mediated by reactivation of RAS/MAPK signaling. Molecular cancer therapeutics, 16(8), 1623-1633.

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Immunoblotting of Oncogenic Signaling

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Cells were seeded in 6-well plates and treated with indicated agents for specified timepoints. Lysates were prepared as previously described, and equal volumes of total cell lysate were processed for immunoblotting10 . Antibodies against phospho-ALK Y1282/1283 (9687), ALK (3633), phospho-AKT S473 (4060), AKT (4691), Phospho-ERK T202/Y204 (9101), ERK (9102), Phospho-Paxillin Y118 (2541), SHP2 (Cell Signaling 3752), and EGFR (4267), were obtained from Cell Signaling Technology and used at 1:1000 dilution. Antibodies against Phospho-EGFR Tyr1068 (AB5644) and Phospho-RSK 359/S363 (AB32413) were purchased from Abcam and used at 1:1000 dilution. GAPDH (MAB374) antibody was purchased from Millipore and used at 1:5000 dilution. K-RAS (F234), H-RAS (F235) and N-RAS (F155) antibodies were purchased from Santa Cruz and used at 1:500 dilution. All secondary antibodies (anti-mouse IgG HRP-linked (7076S) and anti-rabbit IgG HRP-linked (7074S)) were purchased from Cell Signaling and used at 1:50000 dilution.

Dardaei L., Wang H.Q., Singh M., Fordjour P., Shaw K.X., Yoda S., Kerr G., Yu K., Liang J., Cao Y., Chen Y., Lawrence M.S., Langenbucher A., Gainor J.F., Friboulet L., Dagogo-Jack I., Myers D.T., Labrot E., Ruddy D., Parks M., Lee D., DiCecca R.H., Moody S., Hao H., Mohseni M., LaMarche M., Williams J., Hoffmaster K., Caponigro G., Shaw A.T., Hata A.N., Benes C.H., Li F, & Engelman J.A. (2018). SHP2 inhibition restores sensitivity to ALK inhibitors in resistant ALK-rearranged non-small cell lung cancer. Nature medicine, 24(4), 512-517.

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