Nbp1 90654

Manufactured by Cell Signaling Technology

NBP1-90654 is a laboratory reagent produced by Cell Signaling Technology. It is an anti-body used for the detection and analysis of target proteins in experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti citrate synthase, by Cell Signaling Technology (2 mentions) Anti β actin, by Abcam (1 mentions) Anti bnip3, by Cell Signaling Technology (1 mentions) Anti nix, by Cell Signaling Technology (1 mentions) Anti bnip3, by Abcam (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions)

2 protocols using nbp1 90654

Western Blot Analysis of Mitophagy Regulators

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described^{14 (link)}. Antibodies used in this study include: anti-PPTC7 (Novus Biologicals, catalog #NBP1-90654, 1:1000 dilution, 48 h incubation), anti-Citrate synthase (Cell Signaling Technologies, catalog #14309, 1:1000 dilution, overnight incubation), anti-beta actin (Abcam, catalog # ab170325, 1:1000 dilution, overnight incubation), anti-BNIP3 (rodent specific antibody, Cell Signaling Technologies, catalog #3769, 1:1000 dilution, 48 h incubation), anti-NIX (Cell Signaling Technologies, catalog #12396, 1:1000 dilution, overnight incubation), and anti-LC3B (Cell Signaling Technologies, catalog #3868, 1:1000 dilution, overnight incubation). For the immunoprecipitations, the following antibodies were used: anti-BNIP3 (Abcam, clone EPR4034, catalog #ab109362, WB 1:1000), anti-NIX (Cell Signaling Technology, clone D4R4B, catalog #12396, WB 1:1000); anti-FLAG (Sigma-Aldrich, catalog #SAB4301135, WB 1:1000), and anti-vinculin (SCBT, clone G-11; sc-55465, WB 1:1000).

Niemi N.M., Serrano L.R., Muehlbauer L.K., Balnis C.E., Wei L., Smith A.J., Kozul K.L., Forny M., Connor O.M., Rashan E.H., Shishkova E., Schueler K.L., Keller M.P., Attie A.D., Friedman J.R., Pagan J.K., Coon J.J, & Pagliarini D.J. (2023). PPTC7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy. Nature Communications, 14, 6431.

+ Open protocol

+ Expand

Western Blotting of Mitochondrial Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described (Niemi et al., 2019 (link)). Antibodies used in this study include: anti-Pptc7 (Novus Biologicals, catalog #NBP1-90654, 1:1000 dilution, 48 hour incubation), anti-Citrate synthase (Cell Signaling Technologies, catalog #14309, 1:1000 dilution, overnight incubation), anti-beta actin (Abcam, catalog # ab170325, 1:1000 dilution, overnight incubation), anti-Bnip3 (rodent specific antibody, Cell Signaling Technologies, catalog #3769, 1:1000 dilution, 48 hour incubation), and anti-Nix (Cell Signaling Technologies, catalog #12396, 1:1000 dilution, overnight incubation).

Niemi N.M., Serrano L.R., Muehlbauer L.K., Balnis C., Kozul K.L., Rashan E.H., Shishkova E., Schueler K.L., Keller M.P., Attie A.D., Pagan J., Coon J.J, & Pagliarini D.J. (2023). Pptc7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy. bioRxiv.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!