Rat specific horseradish peroxidase polymer anti rabbit antibody

Immunohistochemical staining to evaluate myeloperoxidase (MPO) was performed as described previously (33 (link)). Briefly, paraffin-embedded lung tissue sections were deparaffinized before antigen retrieval. The lung sections were incubated with a solution of 3% H₂O₂ in methanol for 15 min to block endogenous peroxidase. The slides were exposed to rabbit polyclonal antibody (anti-MPO, 1:100, Cell Signaling Technology, Danvers, MA, USA). The slides were then washed and incubated for 30 min with rat-specific horseradish peroxidase polymer anti-rabbit antibody (Nichirei Biosciences, Tokyo, Japan), and then horseradish peroxidase substrate was added for 3 min. The lung sections were then counterstained with hematoxylin.

Lung tissues were subjected to immunohistochemical staining for myeloperoxidase (MPO) using an anti-MPO antibody (1:100; Cell Signaling Technology, Danvers, MA, USA), a rat-specific horseradish peroxidase polymer anti-rabbit antibody (Nichirei Biosciences, Tokyo, Japan), and horseradish peroxidase substrate as previously described [19 (link)]. This was followed by counterstaining using hematoxylin.