Advanced tc

Manufactured by Greiner

Sourced in Germany

The Advanced TC is a versatile laboratory equipment designed for temperature control applications. It features precise temperature regulation capabilities, allowing users to maintain consistent and accurate temperature conditions within their experiments or processes.

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Lab products found in correlation

P mlc, by Cell Signaling Technology (1 mentions) Yap taz, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Advanced TC 24-well plates (2 citations)

2 protocols using advanced tc

Comparative Evaluation of Culture Media

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To compare culture media under 2D culture conditions without coverslips or PuraMatrix, three kinds of media were used: 2% FBS/N2/MEM, DMEM/F12 medium, and basic medium #0. Primary neurons were plated at 3×10⁴ cells/mL into 2D culture vessels, 35-mm glass bottom dishes (Advanced TC, Greiner Bio-One GmbH, Frickenhausen, Germany), and 12-well multiplates (Advanced TC, Greiner Bio-One). The growth area of these vessels was surface-modified for cell culture, primary cell culture in particular.

Kaneko A, & Sankai Y. (2014). Long-Term Culture of Rat Hippocampal Neurons at Low Density in Serum-Free Medium: Combination of the Sandwich Culture Technique with the Three-Dimensional Nanofibrous Hydrogel PuraMatrix. PLoS ONE, 9(7), e102703.

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CDM-Mediated Cytoskeletal Dynamics Analysis

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CDMs were generated on 3.5 cm cell culture dishes Advanced TC (Greiner Bio-One International GmbH, Kremsm€ unster, Austria) without any precoating. Cells were seeded and CDMs were synthesized over a period of 7 days as described before. CDMs were decellularized and WT cells were seeded and incubated for 3 hours. Afterward the dishes were washed with PBS and fixed with 4% PFA. For analyzing Myosin-II-activity, YAP/TAZ nuclear translocation and morphological features immunofluorescence staining was employed as described before. Following antibodies were used: pMLC (#3671, Cell Signaling), pMLC (#3674, Cell Signaling), YAP/TAZ (#8418, Cell Signaling). Fluorescence intensities (gray values) were quantified from images using Fiji ImageJ. For pMLC analysis cell bodies were segmented based on Phalloidin (F-Actin) co-staining. For YAP/TAZ analysis cell nuclei were segmented based on DAPI co-staining.

Maier J.I., Rogg M., Helmstädter M., Sammarco A., Schilling O., Sabass B., Miner J.H., Dengjel J., Walz G., Werner M., Huber T.B, & Schell C. (2021). EPB41L5 controls podocyte extracellular matrix assembly by adhesome-dependent force transmission. Cell reports, 34(12).

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