Dl metanephrine hcl

Manufactured by Merck Group

Sourced in United States

Dl-metanephrine-HCl is a chemical compound used as a reference standard in analytical procedures. It is a hydrochloride salt of dl-metanephrine, which is a metabolite of the neurotransmitter norepinephrine. This compound is typically used in laboratory settings for the identification and quantification of metanephrine in biological samples, such as urine or plasma, using analytical techniques like chromatography or mass spectrometry.

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Lab products found in correlation

Dopamine hcl, by Merck Group (2 mentions) Dl normetanephrine hcl, by Merck Group (2 mentions) L dopa d3, by Merck Group (2 mentions) Stable deuterated isotopesfor dopamine d4 hcl, by CDN Isotopes (2 mentions) Norepinephrine d6 hcl, by CDN Isotopes (2 mentions) Epinephrine d3, by CDN Isotopes (2 mentions) 3 methoxytyramine d4 hcl, by Cambridge Isotopes (2 mentions) Dl metanephrine d3 hcl, by Cambridge Isotopes (2 mentions) 3 methoxytyramine, by Merck Group (2 mentions) Norepinephrine, by Merck Group (2 mentions) Epinephrine, by Merck Group (2 mentions) Propionic anhydride, by Merck Group (1 mentions) Ammonium acetate, by Biosolve (1 mentions) Ammonium hydroxide solution, by Merck Group (1 mentions) In house purification system, by Merck Group (1 mentions) L dopa, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Methanol, by Biosolve (1 mentions) Dipotassium hydrogen phosphate, by Merck Group (1 mentions) Formic acid, by Biosolve (1 mentions)

2 protocols using dl metanephrine hcl

Quantification of Catecholamines and Metabolites

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LC-MS grade acetonitrile, isopropanol, methanol,
formic acid, and ammonium acetate were purchased from Biosolve BV
(Valkenswaard, The Netherlands). Glutathione (reduced), ascorbic acid,
dipotassium hydrogen phosphate, pyridine, and hydrochloric acid (32%)
were from Merck Millipore (Darmstadt, Germany). Ammonium hydroxide
solution (28–30%), propionic anhydride, K₂EDTA dihydrate, l-DOPA, dopamine-HCl, nor epinephrine, epinephrine, 3-methoxytyramine, dl-metanephrine-HCl, dl-normetanephrine-HCl, and l-DOPA-d₃, all of analytical purity,
were purchased from Sigma-Aldrich (MI, USA). Stable deuterated isotopes
for dopamine-d₄-HCl, nor epinephrine-d₆-HCl, and epinephrine-d₃ were from CDN Isotopes (Pointe-Claire, Canada), 3-methoxytyramine-d₄-HCl and dl-metanephrine-d₃-HCl from Cambridge Isotopes (MA, USA), and dl-normetanephrine-d₃-HCl from Medical
Isotopes (NH, USA). Ultrapure water was produced using an in-house
purification system (Merck Millipore, MA, USA).

van Faassen M., Bischoff R., Eijkelenkamp K., de Jong W.H., van der Ley C.P, & Kema I.P. (2020). In Matrix Derivatization Combined with LC-MS/MS Results in Ultrasensitive Quantification of Plasma Free Metanephrines and Catecholamines. Analytical Chemistry, 92(13), 9072-9078.

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Quantification of Plasma Catecholamines and Metanephrines

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Plasma concentrations of free MNs and catecholamines were determined using high-pressure liquid chromatography-tandem mass spectrometry (LC-MS/MS) with online solid-phase extraction, essentially as described by de Jong et al. [7] and van de Merbel et al. [8] . Dopamine-HCl, nor epinephrine, epinephrine, 3-methoxytyramine, DL-metanephrine-HCl, DL-normetanephrine-HCl and L-DOPA-d3, all of analytical purity, were purchased from Sigma Aldrich (MI, USA). Stable deuterated isotopes for dopamine-d4-HCl, nor epinephrine-d6-HCl and epinephrine-d3 were purchased from CDN Isotopes (Pointe-Claire, Canada), 3-methoxytyramine-d4-HCl and DL-metanephrine-d3-HCl from Cambridge Isotopes (MA, USA) and DL-normetanephrine-d3-HCl from Medical Isotopes (NH, USA). All samples were determined in one assay run. Intra-assay coefficients of variability for both plasma concentrations of free MNs and catecholamines were <5%. To show the accuracy of the method, samples from the quality assurance program (QAP) for plasma-free MNs of the Royal College of Pathologists of Australasia (RCPA) were analyzed. From Survey 2019, six samples were analyzed. They revealed excellent agreement with target values reported by the RCPAQAP. Errors ranged from -8.3 to 4% for 3-MT, from -12 to 3.7% for NMN and from -6.6 to 6.5% for MN.

Eijkelenkamp K., van Geel E.H., Kerstens M.N., van Faassen M., Kema I.P., Links T.P, & van der Horst-Schrivers A.N.A. (2020). Blood sampling for metanephrines comparing venipuncture vs. indwelling intravenous cannula in healthy subjects. Clinical chemistry and laboratory medicine, 58(10).

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