CAT: Catalase from bovine liver was purchased from Calbiochem (San Diego, CA), or from Sigma-Aldrich; PAH: Poly(allylamine hydrochloride), MW 120–200 g/mol (for the magnetic region). PAA: Poly(acrylic acid) MW 200,000 g/mol 25% soln. In water (Polysciences). MNP: Magnetic nanoparticles, EMG 705 from FerroTec, 10 nm in diameter particles. BSM: bovine submaxillary mucin (Sigma). JAC: lectin jacalin (Vector Laboratories). TRIS: Tris(hydroxymethyl)aminomethane (Sigma-Aldrich). NHS-Biotin: (+)-Biotin N-hydroxysuccinimide ester (Sigma Aldrich). Positive photoresist S1813, MicroChem. Developer MF319. Micro BCA reagent (Thermo Fisher). 6-Hydroxydophamine (6-OHDA), lipopolysaccharides (LPS), and Triton X-100 were from Sigma-Aldrich (St. Louis, MO, USA). Interferon gamma (INF-γ) was purchased from Peprotech Inc. (RockyHill, NJ).
Interferon gamma inf γ
Manufactured by Thermo Fisher Scientific
Sourced in United States
Interferon gamma (INF-γ) is a protein secreted by immune cells, primarily T cells and natural killer cells. It plays a crucial role in the regulation of the immune response. INF-γ is involved in the activation and enhancement of various immune functions, including the stimulation of macrophages and the upregulation of major histocompatibility complex (MHC) class I and class II molecules.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (1 mentions)
Nhs biotin, by Merck Group (1 mentions)
Bovine submaxillary mucin, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane, by Merck Group (1 mentions)
Microbca reagent, by Thermo Fisher Scientific (1 mentions)
Biotin n hydroxysuccinimide ester, by Merck Group (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Caco 2, by Sartorius (1 mentions)
Interleukin 1 beta il 1β, by Thermo Fisher Scientific (1 mentions)
2 protocols using interferon gamma inf γ
1
Catalase Nanoparticle Functionalization Protocol
2
Stimulation of Intestinal Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human intestinal epithelial cell lines Caco-2 and HCT-116 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were maintained in supplemented EMEM (Caco-2) or RPMI 1640 (HCT116) (both from Biological Industries, Beit HaEmek, Israel), containing 10% FBS, 100 U/ml penicillin and 100 mg/ml streptomycin in a humidified atmosphere of 95% air, 5% CO2 at 37°C. Stimulation with inflammatory cytokines was conducted on 80% confluent cells that were incubated for 6 hours (without serum) with tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) and interferon gamma (INF-γ) (PeproTech, Rocky Hill, NJ, USA) at 10 ng/ml each.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!