PLX5622 (batch#98636) was obtained from MedChemExpress LLC (NJ, USA) and incorporated into Teklad 2018SX Grain-based Chow Diet (at 1.2 g/kg PLX5622) by Research Diets Inc (NJ, USA). 6–7-week-old parkinflx/flx mice were administered the PLX5622 diet, or the control Teklad 2018SX Grain-based Chow Diet, (also supplied by Research Diets Inc) for 20 days. Following this, adult-onset DA-neuron specific parkin deletion was accomplished as described in the previous section. Microglia depletion was assessed via immunoblot and IHC analysis for Iba-1.
+ Open protocol