The largest database of trusted experimental protocols

Anti ccr1

Manufactured by R&D Systems

Anti-CCR1 is a recombinant human CCR1 protein. CCR1 is a G-protein coupled receptor that binds to various chemokines and plays a role in inflammatory processes. The Anti-CCR1 product is a laboratory tool for research purposes.

Automatically generated - may contain errors

2 protocols using anti ccr1

1

Isolation and Characterization of Peritoneal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peritoneal cells were isolated by injecting intraperitoneally 10 ml of Hank's balanced salt solution containing 2% FBS and 1 mM EDTA. The peritoneum was massaged and the fluid was collected and stored on ice. Cells were washed and resuspended in Cell Staining Buffer (BioLegend) containing 10 µg/ml of TruStain fcx (BioLegend) and incubated on ice for 10–15 min. Cells were stained with antibodies against CD11b (M1/70), F4/80 (BM8), B220 (RA3-6B2), Ly6G (1A8), CD45.1 (A20), CD45.2 (104), and CCR1 (643854). All antibodies were purchased from BioLegend except anti-CCR1 which was purchased from R&D Systems. Whole blood was isolated and stained as described. Cells were then washed twice and analyzed on the BD FACS Canto II (BD Biosciences).
+ Open protocol
+ Expand
2

Chemokine Receptor Interaction Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemokine (C-C motif) ligand (CCL) 1 (CCL1), CCL2 and chemokine (C-X-C motif) ligand 12 (CXCL12) were purchased from Protein Foundry (Milwaukee, WI). Antibodies were obtained from Abcam (Cambridge, United Kingdom): anti-α1B-AR (host: rabbit; catalog: ab169523), anti-α1D-AR (host: rabbit; catalog: ab84402), anti-CXCR4 (host: goat, ab1670), anti-HA (host: rabbit; catalog: ab9110) ; LifeSpan Biosciences (Seattle, WA): anti-CCR8 (host: goat; catalog: LS-C187704); R&D Systems (Minneapolis, MN): anti-CCR1 (host: mouse; catalog: MAB145), anti-CCR2 (host: mouse; catalog: MAB48607), allophycocyanin (APC) conjugated anti-mouse CD45 (host: rat; catalog: FAB114A), APC conjugated Immunoglobulin G 2B (IgG2B) isotype control (host: rat; catalog: ICO13A), Immunoglobulin G (IgG) isotype control (host: rabbit; catalog: MAB150), IgG isotype control (host: mouse; catalog: MAB004), and IgG isotype control (host: goat; catalog: AB-108-C); and Sigma-Aldrich (St. Louis, MO): anti- FLAG (host: mouse; catalog: F3165).
Phenylephrine (PE), phentolamine (PT), norepinephrine (NE), lipopolysaccharide (LPS) from Pseudomonas aeruginosa (serotype 10.22; source strain ATCC 27316) and all proximity ligation assay (PLA) reagents were purchased from Sigma-Aldrich.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!