Peritoneal cells were isolated by injecting intraperitoneally 10 ml of Hank's balanced salt solution containing 2% FBS and 1 mM EDTA. The peritoneum was massaged and the fluid was collected and stored on ice. Cells were washed and resuspended in Cell Staining Buffer (BioLegend) containing 10 µg/ml of TruStain fcx (BioLegend) and incubated on ice for 10–15 min. Cells were stained with antibodies against CD11b (M1/70), F4/80 (BM8), B220 (RA3-6B2), Ly6G (1A8), CD45.1 (A20), CD45.2 (104), and CCR1 (643854). All antibodies were purchased from BioLegend except anti-CCR1 which was purchased from R&D Systems. Whole blood was isolated and stained as described. Cells were then washed twice and analyzed on the BD FACS Canto II (BD Biosciences).
Anti ccr1
Manufactured by R&D Systems
Anti-CCR1 is a recombinant human CCR1 protein. CCR1 is a G-protein coupled receptor that binds to various chemokines and plays a role in inflammatory processes. The Anti-CCR1 product is a laboratory tool for research purposes.
Automatically generated - may contain errors
2 protocols using anti ccr1
1
Isolation and Characterization of Peritoneal Cells
2
Chemokine Receptor Interaction Assay
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