Anti mmp1 antibody

Anti-human chemerin antibody and recombinant human chemerin, R&D; Anti-chemerin antibody for immunohistochemistry, Phoenix Pharmaceuticals; Anti-PTEN (mouse mAb), anti-ChemR23, anti-Ub and anti-β-actin antibody, Santa Cruz Biotechnology; Anti-Akt, p-Akt (Thr308), p-Akt (Ser473), phospho-GSK3beta (Ser9), PTEN (rabbit mAb) and p-PTEN (Ser380/Thr382/383) antibodies, Cell Signaling Technology; Anti-HA antibody, Sigma; Myeloperoxidase (MPO) antibody, Abcam; Anti-CMKLR1 antibody, Bioworld Technology Inc; Anti-MMP1 antibody, Proteintech Group; Anti-GST antibody, Sangon Biotech (Shanghai); Rat anti-mouse CD68, AbD Serotect Inc; DeadEnd™ Fluorometric TUNEL System, Promega.

Myr was purchased from Aladdin (Shanghai, China). Myr was dissolved in DMSO (Sigma, St Louis, MO, United States). Fetal bovine serum (FBS), DMEM, trypsin-EDTA (0.25%), and PBS were purchased from Gibco (NY, United States). TNF-α (human recombinant) was obtained from Peprotech (Rocky Hill, NJ, United States). Anti-β-tubulin antibody was obtained from Sigma (St Louis, MO, United States). Anti-AIM2, anti-AKT, and anti-phospho-AKT (Ser473) antibodies were obtained from Cell Signaling Technology (Beverly, MA, United States). Anti-AIM2 antibody for IHC was purchased from Abcam (Cambridge, MA, United States). Anti-IL-6, anti-IL-8, anti-CCL2, anti-MMP-3 and anti-MMP-13 antibodies were obtained from Affinity Biosciences (Jiangsu, China), anti-MMP-1 antibody were obtained from Proteintech (Wuhan, China). Anti-CD11b, anti-FcγRII, anti-FcγRIII and isotype control antibodies were purchased from Biolegend (CA, United States). Red fluorescent beads (particle size: 2 μm) were obtained from Zhongkeleiming (Beijing, China).
Collagen-Type II and complete Freund’s adjuvant were purchased from Chondrex (Woodinville, WA, United States). All materials used in this study were endotoxin-free.