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Tgf β

Manufactured by Neobioscience
Sourced in China

TGF-β is a multifunctional cytokine that regulates various cellular processes, including cell growth, differentiation, and immune function. It is an important signaling molecule involved in the regulation of cellular homeostasis and plays a crucial role in tissue repair and regeneration.

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2 protocols using tgf β

1

Mouse Serum Cytokine Profiling

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For serum samples, peripheral blood samples were collected from mice and left for 2 h at room temperature before centrifuging for 20 min at 2000 g. Serum was removed, and samples were stored at −80 °C. For the cell culture supernatant, precipitates were removed by centrifugation. Mouse TGF-β, IL-17, IL-6, IL-10, TNF-α and IFN-γ (Neobioscience, China) were detected using ELISA kits according to the manufacturer's instructions.
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2

Cytokine Profiling in Mice and Cells

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Brain tissues from different groups of mice were lysed in PBS to obtain homogenates; peripheral blood was drawn into heparin-anticoagulant tubes and then centrifuged to obtain the supernatant, and the supernatant of HL-60 cells subjected to different treatments was also collected. According to the manufacturer’s instructions, TGF-β, TNF-α, IL-10, and IL-1β ELISA kits (Neobioscience, Shenzhen, China) were adopted to detect respective molecule levels in different groups of brain tissue, peripheral blood, and HL-60 cell supernatant samples.
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