Ammonium thiocyanate, lithium chloride (LiCl), poly(methyl methacrylate) (PMMA, Mw ∼350.0 kg mol−1), paraffin wax (mp 53–57 °C), poly (vinyl alcohol) (PVA, Mw 31–35 kg mol−1, 98–99% hydrolyzed), 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), 4′,6‐diamidino‐2‐phenylindole (DAPI), and Dulbecco's Modified Eagle's Medium (DMEM) were purchased from Sigma–Aldrich. Calcium hydroxide was obtained from Fluka Analytical. Dimethylsulfoxide (DMSO) and hydrochloric acid (HCl) were purchased from Merck. 1‐Ethyl‐3‐methylimidazolium acetate ([EMIm][OAc]) was a donation of BASF (Ludwigshafen, Germany). Absolute ethanol was purchased from Fisher Scientific. Analytical grade acetone and tetrahydrofuran (THF) were obtained from VWR. Natal calf serum (NCS) and penicillin‐streptomycin (P/S) were purchased from PAA Laboratories, phosphate buffered saline (PBS) from Life Technologies, and sodium dodecyl sulfate (SDS) from Applichem. NIH 3T3 fibroblast cells were obtained from The Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt 4 6 diamidino 2 phenylindole dapi
Manufactured by Merck Group
Sourced in United States
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a colorimetric assay used to measure cell metabolic activity. 4',6-diamidino-2-phenylindole (DAPI) is a fluorescent stain that binds to DNA, used to detect and quantify nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Polymethyl methacrylate, by Merck Group (1 mentions)
Absolute ethanol, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Lithium chloride (licl), by Merck Group (1 mentions)
Ammonium thiocyanate, by Merck Group (1 mentions)
Paraffin wax, by Merck Group (1 mentions)
Tetrahydrofuran thf, by Avantor (1 mentions)
Penicillin streptomycin p s, by GE Healthcare (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
2 3 4 dihydroxyphenyl ethylamine dopamine hydrochloride, by Merck Group (1 mentions)
Fluorescein isothiocyanate fitc dextran, by Merck Group (1 mentions)
Mirneasy micro kit, by Qiagen (1 mentions)
Phalloidin tritc, by Merck Group (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
5 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt 4 6 diamidino 2 phenylindole dapi
1
Biocompatible Polymer Scaffolds for Cell Culture
2
Formulation and Characterization of Multifunctional Nanocarriers
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D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), coumarin-6 (C6), IR-780, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4',6-diamidino-2-phenylindole (DAPI) and 2-(3,4-dihydroxyphenyl) ethylamine (dopamine) hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Docetaxel (purity: 99.9%) was bought from Shanghai Jinhe Bio-Technology Co., Ltd. (Shanghai, China). Star-shaped copolymer CA-PLGA-b-TPGS was synthesized as described previously 29 (link). All other agents used were of the highest commercial grade available. The human breast carcinoma cell line MCF-7, MDA-MB-231, human prostate adenocarcinoma cell line LNCaP and human embryonic kidney cell line HEK 293 were from American Type Culture Collection (ATCC, Rockville, MD).
3
Aβ25–35 Neurotoxicity Assay
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URB597 3-(3-carbamoylphenyl)-phenyl N-cyclohexylcarbamate was from Selleck Chemicals (Selleck Chemicals, Houston, TX, USA). β-amyloid peptide (Aβ25–35) fragment was synthesized by conventional solid phase chemistry [89 (link)]. Tissue culture medium and serum were from Gibco BRL (Life Technologies Inc., Grand Island, NY, USA). The 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), 4′, 6-diamidino-2-phenylindole (DAPI), and TRITC-phalloidin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fluorescein isothiocyanate (FITC)-dextran was from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Pull-Down and Detection Kits were from Thermo Scientific Pierce, Rockford (Thermo Scientific Pierce, Rockford, IL, USA). The miRNeasy Micro kit was obtained from QIAGEN (Hilden, Germany). The High-Capacity cDNA Reverse Transcription kit and Power SYBR® Green Master Mix was purchased from Applied Biosystems (Foster City, CA, USA).
4
Cell Viability and Apoptosis Assays
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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), antibiotic and antimycotic solution (100X) containing penicillin, streptomycin, amphotericin B, 4,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Tissue-culture plasticware was acquired from Corning (New York, NY, USA), Alexa Fluor 488 Annexin V and the PI/dead cell apoptosis kits (Invitrogen, Life Technologies, Carlsbad, CA, USA), and synthetic CDPs cyclo(-Pro-Val), cyclo(-Pro-Tyr), and cyclo(-Phe-Pro) (G-4730, G-4715, and G-4720, respectively) were acquired from Bachem Co. (Torrance, CA, USA).
5
Nanoparticle-based Drug Delivery Protocol
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Cetyltrimethyl ammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), ammonium hydroxide (NH4OH, 28–30%), sodium hydroxide, succinic anhydride, ammonium molybdate, ascorbic acid, citric acid, NaSiO3·9H2O, sodium dodecyl sulfate (SDS) were obtained from Shan Dong Yu Wang Reagent Company (China). Cy5.5- hydrazide were purchased from Lumiprobe. 4′, 6-diamidino-2-phenylindole (DAPI), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) purchased from Sigma-Aldrich. 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris), pepsase, trypsase, 3-Aminopropyltriethoxysilane (APTES) were obtained from Aladdin. Raw nifedipine (purity > 99.0%) was obtained from Shenyang Funing Pharmaceutical Company (Shenyang, China). All other chemicals were of analytical grade and used without further purification.
Sprague-Dawley (SD) rats (180–220 g) and Kunming mice (18–22 g) were purchased from the Experimental Animal Center of Shenyang Pharmaceutical University. They were acclimated in the controlled environment (temperature: 22 ± 1 °C; humidity: 60 ± 10% and light: 12 h light/dark cycle) with free access to water and commercial laboratory complete food. Animal experiments were carried out in accordance with the Guide lines for Animal Experimentation of Shenyang Pharmaceutical University and the protocol was approved by the Animal Ethics Committee of this institution.
Sprague-Dawley (SD) rats (180–220 g) and Kunming mice (18–22 g) were purchased from the Experimental Animal Center of Shenyang Pharmaceutical University. They were acclimated in the controlled environment (temperature: 22 ± 1 °C; humidity: 60 ± 10% and light: 12 h light/dark cycle) with free access to water and commercial laboratory complete food. Animal experiments were carried out in accordance with the Guide lines for Animal Experimentation of Shenyang Pharmaceutical University and the protocol was approved by the Animal Ethics Committee of this institution.
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