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Hrp conjugated goat anti rabbit igg ha1001

Manufactured by Huabio
Sourced in China

HRP conjugated goat anti-rabbit IgG (HA1001) is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with horseradish peroxidase (HRP) enzyme, which can be used for various immunoassay applications.

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2 protocols using hrp conjugated goat anti rabbit igg ha1001

1

Investigating Role of TNF-α and TL1A in Fibrosis

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The recombinant proteins of human TNF-a (AF-300-01A) and mouse TNF-a (AF-315-01A) were obtained from PeproTech. The pcDNA3.1 and pc-mTL1A were constructed from FengHuiShengWu Biotechnology. Human TL1A siRNA (sc-39846) was obtained from Santa Cruz Biotechnology. TNF-a (ab215188) and collagen I (ab260043) antibodies were obtained from Abcam. Infliximab (A2019) was provided by Selleck Technology. Recombinant human sTL1A (1319-TL) was obtained from RD Systems. Antibodies of a-SMA (#19245), vimentin (#5741), and N-cadherin (#13116) were obtained from Cell Signaling Technology. Fibronectin (ET1702-25), GAPDH (ET1601-4), and HRP conjugated goat anti-rabbit IgG (HA1001) were from HUABIO. DR3 (#PA5-19882) was obtained from Invitrogen Technology. TL1A antibody (DF3053) was constructed by Affinity Biosciences. The HE staining kit (Cat#G1120), Masson staining kit (Cat#G1340), and broad spectrum SP kit (SP0041) were producted from SolarBio.
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2

Molecular Markers in Cell-ECM Interactions

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Lipopolysaccharide (LPS) from Escherichia coli 055:B5 (L2880), 4, 6-diamino-2-phenylindole dihydrochloride (DAPI, 28718-90-3), and fluoresceine isothiocyanate (FITC)-dextran (FD4, 60842-46-8) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-SDC-1 (ET1703-42), anti-Tjp-3 (ER61305), anti-TL1A (ER1917-90), anti-Ctse (ER62913), anti-DR3 (ER65462), anti-GAPDH (ET1601-4), and HRP-conjugated goat anti-rabbit IgG (HA1001) were obtained from HUABIO (Zhejiang, China). Anti-FITC (ab19224), anti-prosurfactant protein C (SP-C, ab90716), anti-SDC-1 (ab128936), recombinant human MMP9 (ab168863), and Ctse activity assay kit (ab211081) were obtained from Abcam (Cambridge, UK). Anti-DR3 (PA5-19882) and anti-Tjp-3 (#36-4100) were obtained from Thermo Scientific (Waltham, US). Recombinant human active heparanase/HPA protein (7570-GH) and Ctse protein (1294-AS) were obtained from RD Biotechnology Ltd. (Franche-Comte, France). The BCA protein assay kit (PC0020), hematoxylin–eosin (HE) stain kit (G1120), and broad spectrum SP kit (SP0041) were sourced from Solarbio (Beijing, China). Anti-Tjp-3 (#3704) was obtained from Cell Signal Technology (Danvers, MA, USA). Dylight 488 goat anti-mouse IgG (A23210) and goat anti-rabbit IgG (A23220), Dylight 549 goat anti-mouse IgG (A23310), and goat anti-rabbit IgG (A23320) were sourced from Abbkine (Wuhan, China).
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