Mirna 4677 3p mimics or control

For the analysis of LINC00540 and miRNA-4677-3p interactions, sorafenib-resistant cells were seeded into 24-well plates (6 × 10⁴ cells/well). The cells were co-transfected with LINC00540-WT-pesicheck2, LINC00540-MUT-pesicheck2 (FenghBio, Changsha, China), or miRNA-4677-3p mimics (or Control) (GenePharma, Co., Ltd, China) using TransIntroTM EL Transfection Reagent (TransGen Biotech, Beijing, China). Similarly, for the analysis of AKR1C2 and miRNA-4677-3p interactions, cells were co-transfected with AKR1C2-WT-pesicheck2 (or MUT) (FenghBio) and miRNA-4677-3p mimics (or Control) (GenePharma, China) using TransIntroTM EL Transfection Reagent. Luciferase activity was measured after 48 h of co-transfection using the TransDetect Double-Luciferase Reporter Assay kit (Transgen Biotech).