Alexa fluor 647 anti mouse cd8a

The samples of well digested tumor suspension were added with 1 μL (0.1 mg·mL⁻¹) of fixable viability stain (BD, 564406) in each tube and incubated for 15 min at room temperature. All samples were then blocked with 2 μL Fc blocking solution (0.5 mg·mL⁻¹) at 4 °C for 5 min, washed and then incubated with the following specific antibodies on ice for 30 min: anti‐mouse CD16/CD32 (BD, 553141), APC‐Cy™7 anti‐mouse CD45 (BD, 561037), APC anti‐mouse CD11b (BioLegend, San Diego, CA, USA, 101212), PE anti‐mouse F4/80 (BioLegend, 123110), PE‐Cy™7 anti‐mouse CD86 (BD, 560582), PE anti‐mouse CD80 (BD, 561955), Alexa Fluor® 488 anti‐mouse CD206 (BioLegend, 141710), FITC anti‐mouse CD4 (BD, 553046), Alexa Fluor® 647 anti‐mouse CD8a (BioLegend, 100724), PE‐Cy™7 anti‐mouse Ly‐6G and Ly‐6C (BD, 552985). The analysis was performed by Beckman CytoFlex S (Brea, CA, USA).