R23860

Paraffin-embedded DLBCL sections confirmed by pathologists were collected. Paraffin-embedded DLBCL sections were deparaffinized with the dewaxing agent and absolute ethanol. After that, citric acid solution (Servicebio, G1202) was used for antigen retrieval and 3% H2O2 was used to inactivate endogenous peroxidase. Then 3% bovine serum albumin (Servicebio, GC305010) was added to block sections for 30 minutes. Add the primary antibody, anti-CD79A (ZEN BIO, R23860), and incubate overnight at 4 °C. After washing sections three times using PBS, add goat anti-rabbit IgG H&L (HRP) (Servicebio, GB21303) and incubate for 50 minutes. After washing three times using PBS, add CY3-tyramide (Servicebio, G1223) for 10 minutes. Wash with a citric acid solution to remove the first type of primary antibody. Then add the second primary antibody, anti-HMGB3 (Affinity Biosciences, AB_2841269), and incubate overnight at 4 °C. After washing sections three times using PBS, add anti-rabbit IgG (Alexa Fluor 488 conjugate) (Servicebio, GB25303) and incubate for 50 minutes. Finally, sections were stained with DAPI (Servicebio, G1012) and sealed with an anti-fluorescent quenching agent (Servicebio, G1401). Finally, fluorescence image capture was performed using laser scanning confocal microscopy and processed using ImageJ software.